肠壁穿刺逆行胰胆管注射牛磺胆酸钠重症急性胰腺炎造模52例分析

Establishing severe acute pancreatitis model on SD rats by retrograde injection of sodium taurocholate into biliopancreatic duct through duodenal wall: An analysis of 52 cases

目的:探讨肠壁穿刺逆行胰胆管注射牛磺胆酸钠造模过程中的操作注意事项,以提高造模成功率。方法:52例SD大鼠通过肠壁穿刺逆行胰胆管注射牛磺胆酸钠建立重症急性胰腺炎模型。术后24h取胰腺组织行HE染色,判断模型是否成功。结果:52例中造模成功38例,占73.1%,24h成活率86.5%。结论:在造模过程中注意麻醉深度、穿刺角度等问题可以有效提高造模成功率,减少死亡率。

Objective： To investigate the points for attention in establishing severe acute pancreatitis （SAP） model by retrograde injection of sodium taurocholate into biliopancreatic duct through duo denal wall. Methods： 52 cases of SAP rat models were established taurocholate into biliopancreatic duct through duodenal wall. 24 h by af retrograde injection of sodium ter SAP induction, pancreatic tissues were performed HE staining to judge whether the models were successful or not. Results： Of the 52 cases, the successful rate the established models was 73.1%, and the survival rate after 24 hrs was 86.5%. Conclusion： During the experiment, attention should be paid to the depth of anesthesia, the puncture angle, etc, in order to increase success rate and decrease the mortality rate of establishing the models.