期刊文献+

MMP-9-1562位点T等位基因与儿童EV71重症脑炎易感性关系的研究(英文) 被引量:2

Association of Functional-1562 C/T Polymorphism in Matrix Metalloproteinase-9(MMP-9) with Risk of Severe Encephalitis in Enterovirus 71 Infected Children
原文传递
导出
摘要 目的:研究基因MMP-9-1562位点基因多态性与肠道病毒71型(EV71)脑炎的关系,探讨不同基因型对EV71脑炎患病风险及病情程度的影响。方法:运用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)检测EV71感染阳性病毒性脑炎患儿及正常对照组儿童基因组MMP-9-1562位点碱基变异情况。结果:EV71脑炎重症组MMP-9-1562位点T等位基因频率分布明显高于EV71脑炎轻症组(16%VS 7.5%,OR 2.362,95%CI 1.170~4.768,P〈0.05)。MMP-9-1562位点T等位基因携带者感染EV71后可明显增加神经系统并发症的发生率。EV71脑炎患儿MMP-9-1562位点T等位基因频率分布与正常对照儿童比较无明显差异。结论:MMP-9-1562位点T等位基因携带者在感染EV71后易发展为重症中枢神经系统感染;MMP-9-1562位点T等位基因可增加EV71感染后神经系统并发症的发生率;MMP-9-1562位点单核苷酸多态性与EV71脑炎的发病率无关。 Objective: To investigate the relationship between the polymorphisms of matrix metalloproteinase-9(MMP-9)-1562 and the susceptibility of enterovirus71 encephalitis,and explore the risk factors of EV71 encephalitis.Methods: Enterovirus 71 in the feces and cerebrospinal fluid of encephalitis children was detected by reverse transcription polymerase chain reaction(RT-PCR),and EV71 specific primer was used PCR-restriction fragment length polymorphism(PCR-RFLP) techniques were performed to determine the genotypes of MMP-9 gene in encephalitis group and in control group.Results: The MMP-9-1562 T allele was observed with sigificantly greater frequency in patients with serious EV71 encephalitis compared with slight EV71 encephalitis patients(16 % VS 7.5 %,OR 2.362,95 % CI 1.170~4.768,P0.05).T alleles carriers of MMP-9-1562 can increase the incidence of nervous complications after infection EV71.However,for the allele distribution of the MMP-9-1562C/T polymorphism,there was no significant difference between EV71 encephalitis patients and healthy children.Conclusion: MMP-9-1562 T allele carriers in EV71 infection may develop into severe central nervous system(CNS) infection.T alleles of MMP-9-1562 can increase the incidence of nervous complications after EV71 infection,but the MMP-9-1562C/T polymorphism is not associated with risks of either EV71 encephalitis or healthy children.
出处 《现代生物医学进展》 CAS 2013年第15期2870-2874,共5页 Progress in Modern Biomedicine
基金 National Science Foundation of China(31171212)~~
关键词 肠道病毒71型(EV71) 脑炎 金属蛋白酶-9(MMP-9) 基因多态性 Enterovirus71 Encephalitis MMP-9 Polymorphisms
  • 相关文献

参考文献20

  • 1AbuBakar S, Sam IC, Yusof J,et al. Enterovirus 71 outbreak[J] .Brunei.Emerg Infect Dis,2009,15:79-82.
  • 2Weng KF, Chen LL, Huang PN,et al. Neural pathogenesis ofenterovirus 71 infection[J]. Microbes Infect,2010,12:505-510.
  • 3Yang F, Ren L, Xiong Z,et al. Enterovirus 71 outbreak in the Peopler sRepublic of China in 2008[J]. J Clin Microbiol,2009,47:2351-2362.
  • 4Yang J, Zhao N,Chen ZB,et al.Association of interleukin 10 andinterferon gamma gene polymorphisms with enterovirus 71encephalitis in patients with hand, foot and mouth disease [J].Scandinavian Journal of Infectious Diseases, 2012,Early Online:1-5.
  • 5Kehle J, Roth B, Metzger C,et al. Molecular characterization of anenterovirus 71 causing neurological disease in Germany [J]. J.Neurovirol,2003,9:126-128.
  • 6Schuffenecker I,Mirand A, Antona D, et al. Epidemiology of humanenterovirus 71 infections in France, 2000-2009 [J]. J Clin Virol,2011,50:50-56.
  • 7Cunningham LA, Wetzel M, Rosenberg GA, et al. Multiple roles forMMPs and TIMPs in cerebral ischemia[J]. Glia,2005,50(4):329-339.
  • 8Gasche Y, Soccal PM, Kanemitsu M, et al. Matrix metalloproteinasesand diseases of the central nervous system with a special emphasis onischemic brain[J]. Front Biosci,2006,11:1289-1301.
  • 9Zhang B, Henney A, Eriksson P, et al. Genetic variation at the matrixmetalloproteinase-9 locus on chromosome 20ql2.2-13.1 [J]. HumGenet,1999,105:418-423.
  • 10陈宗波,付振荣,吴福玲,隋爱华,杨堃,刘小梅,钱娜,赵娜,陈真真.柯萨奇B3病毒VP1序列变异与小儿中枢神经系统损害关系的探讨[J].中华儿科杂志,2010,48(4):268-272. 被引量:7

二级参考文献17

  • 1侯晓君,谢文煌.重症病毒性脑炎患儿的磁共振影像特点[J].中国实用儿科杂志,2006,21(7):537-538. 被引量:18
  • 2Oberste MS,Maher K,Kennett ML,et al.Molecular epidemiology and genetic diversity of echovirus type 30(E30):genotypes correlate with temporal dynamics of E30 isolation.J Clin Microbiol,1999,37:3928-3933.
  • 3Mirand A,Archimbaud C,Henquell C,et al.Prospective identification of HEV-B enteroviruses during the 2005 outbreak.J Med Virol,2006,78:1624-1634.
  • 4Singh S,Chow VT,Chan KP,et al.RT-PCR,nucleotide,amino acid and phylogenetic analyses of enterovirus type 71 in Asia J Virol Methods,2000,88:193-204.
  • 5Norder H,Bjerregaard L,Magnius LO.Homotypic echoviruses share aminoterminal VP1 sequence homology applicable for typing.J Med Virol,2001,63:35-44.
  • 6Iturriza-Cómara M,Megson B,Gray J.Molecular detection and characterization of human enteroviruses directly from clinical samples using RT-PCR and DNA sequencing.J Med Virol,2006,78:243-253.
  • 7KOMATSU K.Outbreak of severe neurologic involvement associated with enterovirus 71 infection[J].Pediatr Neurol,1999,20:17-23.
  • 8CHEVILLARD C,HENRI S,STEFANI F,el al.Two new polymorphisms in the human interferon gamma (IFN-gamma) promoter[J].Eur J Immunogenet,2002,29:53-56.
  • 9LIO D,MARINO V,SERAUTO V,el al.Genotype frequencies of the +874 T/A single nueleotide polymorphism in the first intron of the interferon-γ gene in a sample of Sicilian patients affected by tuberculosis[J].Eur J Immunogenet,2002,29:371-374.
  • 10BEN-ARI Z,MOR E,PAPO O,et al.Cytokine gene polymorphisms in patients infected with hepatitis B virus[J].Am J Gustroenterol,2003,98:144-150.

共引文献15

同被引文献22

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部