摘要
目的:研究基因MMP-9-1562位点基因多态性与肠道病毒71型(EV71)脑炎的关系,探讨不同基因型对EV71脑炎患病风险及病情程度的影响。方法:运用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)检测EV71感染阳性病毒性脑炎患儿及正常对照组儿童基因组MMP-9-1562位点碱基变异情况。结果:EV71脑炎重症组MMP-9-1562位点T等位基因频率分布明显高于EV71脑炎轻症组(16%VS 7.5%,OR 2.362,95%CI 1.170~4.768,P〈0.05)。MMP-9-1562位点T等位基因携带者感染EV71后可明显增加神经系统并发症的发生率。EV71脑炎患儿MMP-9-1562位点T等位基因频率分布与正常对照儿童比较无明显差异。结论:MMP-9-1562位点T等位基因携带者在感染EV71后易发展为重症中枢神经系统感染;MMP-9-1562位点T等位基因可增加EV71感染后神经系统并发症的发生率;MMP-9-1562位点单核苷酸多态性与EV71脑炎的发病率无关。
Objective: To investigate the relationship between the polymorphisms of matrix metalloproteinase-9(MMP-9)-1562 and the susceptibility of enterovirus71 encephalitis,and explore the risk factors of EV71 encephalitis.Methods: Enterovirus 71 in the feces and cerebrospinal fluid of encephalitis children was detected by reverse transcription polymerase chain reaction(RT-PCR),and EV71 specific primer was used PCR-restriction fragment length polymorphism(PCR-RFLP) techniques were performed to determine the genotypes of MMP-9 gene in encephalitis group and in control group.Results: The MMP-9-1562 T allele was observed with sigificantly greater frequency in patients with serious EV71 encephalitis compared with slight EV71 encephalitis patients(16 % VS 7.5 %,OR 2.362,95 % CI 1.170~4.768,P0.05).T alleles carriers of MMP-9-1562 can increase the incidence of nervous complications after infection EV71.However,for the allele distribution of the MMP-9-1562C/T polymorphism,there was no significant difference between EV71 encephalitis patients and healthy children.Conclusion: MMP-9-1562 T allele carriers in EV71 infection may develop into severe central nervous system(CNS) infection.T alleles of MMP-9-1562 can increase the incidence of nervous complications after EV71 infection,but the MMP-9-1562C/T polymorphism is not associated with risks of either EV71 encephalitis or healthy children.
出处
《现代生物医学进展》
CAS
2013年第15期2870-2874,共5页
Progress in Modern Biomedicine
基金
National Science Foundation of China(31171212)~~