B细胞性非霍奇金淋巴瘤中CHFR mRNA的表达与其临床病理特征关系的研究

The Relationship between CHFR mRNA Expression and Clinic-pathological Features in B-cell Non-Hodgkin's Lymphoma

目的:探讨CHFR(checkpoint with FHA and ring finger)基因在B细胞非霍奇金淋巴瘤(B-NHL)中的表达水平及其与临床病理特征之间的关系。方法:采用荧光定量PCR方法检测81例B细胞非霍奇金淋巴瘤组织和38例淋巴结反应性增生组织(RH)CHFR mRNA的表达水平。结果:①81例B-NHL组织中低水平表达CHFR mRNA,其中2例表达缺失;而在淋巴结反应性增生组中该基因全部表达,差异有统计学意义(P<0.05)。②B-NHL患者中I+II期组和III+IV期组CHFR mRNA表达存在差异,III+IV期表达水平低于I+II期,差异有统计学意义(P<0.05);B-NHL组按IPI评分分0-1分组和2-5分组,高评分组CHFR mRNA表达量低于低评分组,差异具有统计学意义(P<0.05);而CHFR mRNA表达量与年龄、性别、有无症状、LDH水平及ECOG评分无相关性。结论:CHFR表达水平的降低在非霍奇金淋巴瘤的发生过程中具有一定作用,并与其恶性程度及进展相关。

Objective： To investigate the CHFR（checkpoint with FHA and ring finger） expression in non-Hodgkin lymphoma（NHL）,and the relationship between CHFR expression and clinic-pathological characteristics.Methods： The expression of CHFR mRNA were detected in the tissues of 81 patients with B-NHL as well as 38 reactive hyperplasia of lymph node（RH） by Real-time fluorescence quantitative PCR.Results： ①CHFR mRNA was shown in the RH group,but the level of mRNA expression was significantly lower in 81 B-NHL examples,and the mRNA expression was loss in 2 patients,the discrepancy had statistical significance（P0.05）.②The expression was different between B-NHL patients in I＋II group and III＋IV group（P0.05）.B-NHL was divided into 0-1 group and 2-5 group according to IPI score,the expression of CHFR mRNA in high score group was lower than that in the low score group（P0.05）.However,the expression of CHFR mRNA had no correlation with clinicpathological characteristics,such as age,gender,B symptoms,serum LDH and ECOG.Conclusion： The down-regulation of CHFR mRNA expression may contribute to the occurrence of B-NHL,and is associated with the malignancy and progress of B-NHL.