MR对小鼠原代棕色脂肪细胞功能的影响

Role of MR in the function of primary brown adipocyte in mouse

目的:使用小鼠原代棕色脂肪细胞,研究盐皮质激素受体(mineralocorticoid receptor,MR)对其功能的影响。方法 :使用MR激动剂醛固酮、MR拮抗剂螺内酯、糖皮质激素可的松分别作用于小鼠原代棕色脂肪细胞,观察棕色脂肪细胞相关基因的改变。结果:MR的配体(盐皮质激素)可显著增加其功能基因非偶联蛋白1(uncoupling protein-1,UCP1)、细胞死亡诱导DNA碎片因子-α-样效应结合区域蛋白(the cell death-inducing DNA fragmentation factor-α-like effector domain-containing protein,CIDEA)、过氧化酶增殖活化受体α(peroxisome proliferator-activated receptor alpha,PGC1α)的表达,同时也促进分化基因脂肪酸结合蛋白4(fatty acid binding protein 4,FABP4)的表达,相反MR的拮抗剂可抑制棕色脂肪细胞功能基因的表达。另一方面,盐皮质激素还可以协同糖皮质激素共同调节棕色脂肪细胞功能基因UCP1的变化。结论:MR是调控原代棕色脂肪细胞功能的重要因子。

Objective：To investigate the role of mineralocorticoid receptor （MR）in regulating the function of mouse primary brown adipocyte. Methods：The primary brown adipoeytes were separated from C57BL/SJ mice and induced for differentiation. The expression of genes related to brown fat function, including uncoupling protein-1 （UCPI）, the cell death-inducing DNA fragmentation factor-a-like effector, domain- containing protein （CIDEA）, peroxisome proliferator-activated receptor alpha （PGC let） and fatty acid binding protein 4 （FABP4） were observed in MR ligands （aldosterone）, MR antagonist （spironolactone） and glucocorticoids （cortisone） treated cells, respectively. Results：Mineralocorticoid could significantly increase the expressions of UCP1,PGClct and FABP4. Conversely, the expression of UCP1 was suppressed by MR antagonists. Moreover, MR regulated the brown adipocyte function with the interaction of glueocortieoid. Conclusion： MR plays an important role in the function of primary brown adipocvte.