HPLC测定异黑成熟颗粒中田蓟苷和甘草酸的含量

Content Determmination of Tilianin and Glycyrrhizic Acid in Abnormal Savda Munziq Granules by HPLC

目的:建立测定异黑成熟颗粒中2种有效成分田蓟苷和甘草酸含量的方法。方法:采用高效液相色谱法,Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.5%甲酸(27∶73)为流动相,检测波长324 nm,柱温35℃,流速1.0 mL.min-1。Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-0.2 mol.L-1酸铵-冰醋酸(67∶33∶1)为流动相,检测波长250 nm,柱温30℃,流速1.0 mL.min-1。结果:田蓟苷和甘草酸分别在1.14～114μg(r=0.999 9)和20.6～206μg(r=0.999 7)线性关系良好;平均回收率分别为98.26%(RSD 2.29%),99.39%(RSD 1.86%)。结论:该方法简便可行,重复性好,可用于异黑成熟颗粒中田蓟苷和甘草酸含量测定。

Objective： To establish the method for the content determination of tilianin and glycyrrhizic acid in Abnormal Savda Munziq granules.Method： HPLC was adopted to determine the content of tilianin and glycyrrhizic acid.The column for Tilianin is Diamonsil C18（4.6 mm ×250 mm,5 μm）.The mobile phase was consisted of acetonitrile-0.5% formic acid（27 ∶ 73） with the detection wavelength at 324 nm.The column temperature was set at 35 ℃ and the flow rate was set at 1.0 mL.min-1.The column for glycyrrhizic acid was Diamonsil C18（4.6 mm ×250 mm,5 μm）.The mobile phase was consisted of methanol-0.2 mol.L-1 ammonium acetate-glacial acetic acid（67∶ 33∶ 1） with the detection wavelength at 250 nm.The column temperature was kept at 30 ℃ and the flow rate was set at 1.0 mL.min-1.Result： There was good linearity between the peak area and concentration within the range of 1.14-114 μg（r = 0.999 9） for tilianin and 20.6-206 μg（r = 0.999 7） for glycyrrhizic acid respectively.Conclusion： The method is simple,accurate and specific for quality control of Abnormal Savda Munziq granules.