摘要
目的:研究大血藤药材的UPLC指纹图谱。方法:采用色谱法,Agilent ZORBAX RRHD Eclipse Plus C18色谱柱(2.1 mm×100 mm,1.8μm),洗脱系统0.05%乙酸水-甲醇梯度洗脱,检测波长280 nm,流速0.3 mL.min-1,柱温35℃。测定10批大血藤、2批鸡血藤及1批五香血藤的指纹图谱。结果:建立了大血藤药材UPLC指纹图谱分析方法,确立了19个共有峰,指认了4个峰。10批大血藤药材相似度>0.837,大血藤与混淆品(鸡血藤和五香血藤)的指纹图谱具有明显差异。结论:建立的方法快速、准确、重复性好,为评价和控制大血藤药材的质量提供参考。
Objective： To establish a HPLC fingerprint method of Sargentodoxa cuneata.Method： Chromatography was carried out on a Agilent ZORBAX RRHD Eclipse Plus C18column（2.1 mm × 100 mm,1.8 μm） with gradient eluting system of 0.05% acetic acid water-methanol at a flow rate of 0.3 mL.min-1,detection wavelength was 280 nm,column temperature was at 35 ℃.Ten batches of S.cuneata,two batches of S.suberectus and a batch of Schisandra sphenunthera were determined for similarity comparison.Result： UPLC fingerprint analysis method of S.cuneata was established,there were 19 common peaks,four of them were identified.Similarity of 10 batches S.cuneata was more than 0.837,and fingerprint of S.cuneata had a significant difference to S.suberectus and S.sphenunthera.Conclusion： This method was rapid,accurate and reproducible,it could provide a reference for quality evaluation and control of S.cuneata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期108-111,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省优秀科技教育人才省长专项基金(黔省专合字[2011]60号)
