HPLC同时测定牛黄消炎灵胶囊中3种药效成分的含量

Determination of Three Active Components in Niuhuang Xiaoyanling Capsule by HPLC

目的:建立高效液相色谱同时测定牛黄消炎灵胶囊中栀子苷、盐酸小檗碱和黄芩苷3种药效成分含量的方法。方法:采用固定相为Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),以乙腈-0.2%磷酸水溶液为流动相进行梯度洗脱,流速0.8～1.0 mL.min-1,柱温30℃,双波长紫外检测。结果:栀子苷、盐酸小檗碱、黄芩苷的进样量分别在0.020 4～0.408μg(r=0.999 7),0.041 2～0.412μg(r=0.999 8),0.060 8～0.608μg(r=0.999 9)与其峰面积积分值呈良好的线性关系,平均回收率分别为99.12%,99.92%,99.32%,RSD分别为1.77%,1.24%,1.63%。结论:方法操作简便,准确度高,重复性好,专属性强,可用于牛黄消炎灵胶囊的质量控制。

Objective： To develop a reversed-phase HPLC method for the determination of three active components including geniposide,berberine hydrochloride and baicalin in Niuhuang Xiaoyanling capsule.Method： The determination was performed on a ZORBAX Eclipse XDB-C18 column（4.6 mm × 150 mm,5 μm） with acetonitrile-0.2% phosphoric acid as the mobile phase by gradient elution at the flow rate of 0.8-1.0 mL.min-1.The detection wavelength was set at 240 nm for geniposide and hydrochloride baicalin,278 nm for berberine respectively.The column temperature was maintained at 30 ℃.Result： The calibration curves of geniposide,berberine hydrochloride and baicalin were linear in the ranges of 0.020 4-0.408 μg（r = 0.999 7）,0.041 2-0.412 μg（r = 0.999 8）,0.060 8-0.608 μg（r = 0.999 9） respectively.The average recoveries were 99.12%,99.92% and 99.32% respectively;RSDs were 1.77%,1.24%,1.63% respectively.Conclusion： This proposed method is simple,accurate and reproducible,and suitable for the determination of the content of three analytes in Niuhuang Xiaoyanling capsule;it can be also used for quality control and evaluation of related capsule.