不同产地银杏叶中总银杏酸的含量比较

Comparative Analysis of Total Ginkgolic Acids in Ginkgo biloba Leaves of Different Habitats

目的:比较不同产地银杏叶中总银杏酸的含量。方法:采用HPLC测定银杏叶中总银杏酸的含量。色谱条件为依利特Hypersil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-1%冰醋酸水溶液(90∶10),流速1.0 mL.min-1,检测波长310 nm,柱温35℃,进样量20μL,外标法计算含量。结果:各产地银杏叶中总银杏酸含量差异较大,山东郯城总银杏酸含量最高(2.38%),江苏邳州最低(1.28%)。结论:不同产地银杏叶中总银杏酸含量差异明显,应选择使用。

Objective： To study the change of total ginkgolic acids in Ginkgo biloba leaves of different habitats.Method： The content of total ginkgolic acids in G.biloba leaves was determined by HPLC.A Hypersil C18column（4.6 mm × 250 mm,5 μm） and the mobile phase of methanol and 1% acetic acid（90 ∶ 10） were used;the flow rate was 1.0 mL.min-1,the wavelength was set at 310 nm.The content was calculated with external standard method.Result： The content of total ginkgoic acids in G.biloba leaves varied with different habitats.The content in the samples from Tancheng was the highest,while Pizhou is the lowest.Conclusion： The content of total ginkgolic acids in G.biloba leaves from different habitats is markedly different.We should have a choice in use.