三苦滴丸抗急性心肌缺血拆方研究

Experimental Study on Protection of Separated Recipe of Sanku Dropping Pills on Acute Myocardial Ischemia

目的:通过观察三苦滴丸拆方对急性心肌缺血动物模型的影响,寻找其组方三七和苦碟子的最佳配比剂量。方法:将Wistar大鼠随机分为模型组、三苦滴丸2∶8(三七∶苦碟子)高剂量组、3∶7高剂量组、4∶6高剂量组、2∶8中剂量组、3∶7中剂量组、4∶6中剂量组和2∶8组低剂量、3∶7组低剂量、4∶6低剂量组给药组,连续ig给药7 d,ip异丙肾上腺素20 mg.kg-1连续注射3 d诱导急性心肌缺血动物模型,观察各组动物心电图S-T段变化(mV)、血清中心肌酶及心肌组织中氧自由基相关指标的变化。结果:三苦滴丸各比例组均可降低模型动物心电图ST段变化(P<0.05,P<0.01,P<0.001)和血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)含量(P<0.05,P<0.01),提高心肌组织中超氧化物歧化酶(SOD)活性,减少丙二醛(MDA)含量(P<0.05,P<0.01),但三苦滴丸3∶7组与4∶6组各剂量其作用效果及发挥时效都不及三苦滴丸2∶8组各剂量。结论:三苦滴丸中三七与苦碟子最佳比例确定为2∶8。

Objective： To find the best matching dosage of Panax notoginseng and Ixeris sonchifolia Hance in Sanku dropping pills through observing the effect of its separated recipe on a acute myocardial ischemia.Method： The health Wistar rats were randomly divided into model group,Sanku dropping pills 2 ∶ 8（P.notoginseng∶I.sonchifolia） high-dose group,3∶ 7 high-dose group,4∶ 6 high-dose group,2∶ 8 middle-dose group,3∶ 7 middle-dose group,4∶ 6 middle-dose group,2∶ 8 low-dose group,3∶ 7 low-dose group,4∶ 6 low-dose group,ig administration lasted 7 days.The animal model of acute myocardial ischemia was reduced by continuous intraperitoneal injection isopropylarterenol（20 mg.kg-1） during three days and observe the change of electrocardiogram S-T,myocardial enzyme in blood serum,and the index related to oxygen free radicals in myocardial tissue were observed.Result： It showed that,all the proportion group of Sanku dropping pills could decrease electrocardiogram ST（P ＆lt; 0.05,P ＆lt; 0.01,P ＆lt; 0.001） and the value of lactic clehydrogense（LDH） and creatine kinase（CK） in blood serum（P ＆lt; 0.05,P ＆lt; 0.01）,increase the activity of superoxide dismutase（SOD） and lessen the value of malondi aldehyde MDA in myocardial tissue（P ＆lt;0.05,P ＆lt;0.01）,but the effect and educe seasoning of dosage of 3∶ 7 and 4∶ 6 groups were inferior to dosage of 2∶ 8 groups.Conclusion： The best matching dosage of P.notoginseng.and I.Sonchifolia in Sanku dropping pills were 2∶ 8.