虎杖水提和乙醇提取物抗抑郁作用的比较

Antidepressant Effect of Extracts from Rhizome of Polygonum cuspidati

目的:考查虎杖水提物(HZ-W)和虎杖75%乙醇提取物(醇提物,HZ-E)的抗抑郁作用。方法:雄性昆明种小鼠随机分为模型组、盐酸氟西汀(FH,10 mg.kg-1)、HZ-W(1.5,3 g.kg-1)和HZ-E(1.5,3 g.kg-1)组,连续灌胃(ig)给药7 d,1次/d,末次给药后1 h,进行悬尾实验(TST)、强迫游泳实验(FST)和开场实验(OFT)测试。结果:模型组小鼠TST和FST不动时间分别为(95.83±17.18),(126.00±20.79)s,HZ-W低剂量和高剂量、HZ-E高剂量以及FH均显著缩短了不动时间(P<0.05),而HZ-E低剂量对此无显著影响。OFT中,模型组小鼠3 min内穿格次数为(88.44±19.22)次,各给药组与之相比均无显著差异。结论:首次证实HZ-W和HZ-E均具有抗抑郁活性,且以HZ-W作用更强。

Objective： To investigate the antidepressant effect of extracts of the water（HZ-W） and 75% ethanol（HZ-E） from rhizome of Polygonum cuspidatum Sieb.et Zucc.Method： Mice were treated with HZ-W（1.5,3 g.kg-1）,HZ-E（1.5,3 g.kg-1）,and fluoxetine hydrochloride（FH,10 mg.kg-1） everyday for seven consecutive days by intragastric administration（ig）.The mouse forced swim test（FST）,tail suspension test（TST） and open field test（OFT）,were used to observe antidepressant activities of HZ-W and HZ-E,respectively,at 1 h after the last administration.Result： The immobility time of TST and FST in model mice was（95.83 ±17.18）,（126.00 ±20.79） s respectively and there was significant decrease among HZ-W（1.5,3 g.kg-1）,HZ-E（3 g.kg-1）,FH（10 mg.kg-1） groups compared with model group.The numbers of crossing squares in 3 min in model mice during OFT were（88.44 ± 19.22）,and there was no significant difference among HZ-W,HZ-E,FH groups compared with model group.Conclusion： The present study firstly indicates that HZ-W and HZ-E exerts antidepressant activity and the former is more potent than the latter at the same dose.