三九胃泰方抗胃溃疡有效部位的研究

Study on the Effective Fractions of Sanjiu Weitai Prescription for Anti-gastric Ulcer Activity

目的:对三九胃泰方抗胃溃疡的有效部位进行筛选。方法:将SD大鼠分为正常对照组、模型组、阳性对照(西米替丁(0.1 g.kg-1)与三九胃泰颗粒剂(2 g.kg-1)组、石油醚萃取物组、醋酸乙酯萃取物组、正丁醇萃取组及水萃取组,按生药剂量均为2 g.kg-1ig给药7 d,采用乙醇造大鼠胃黏膜损伤模型,以胃溃疡抑制率、胃蛋白酶活力、血清中丙二醛(MDA)含量与超氧化物歧化酶(SOD)活力为检测指标,对不同极性溶剂(石油醚、醋酸乙酯、正丁醇与水)萃取部位的抗胃溃疡活性进行评价。结果:三九胃泰方各极性萃取部位均能显著抑制乙醇致大鼠胃黏膜损伤(P<0.05),其中石油醚萃取部位胃溃疡抑制率最高(89.15%),高于西米替丁组(59.90%)及三九胃泰颗粒剂组(55.31%)。三九胃泰方各极性萃取部位均可显著降低大鼠胃蛋白酶活力,同时增强血清中SOD的活力(P<0.05)。结论:三九胃泰方不同极性萃取部位均具有显著的抗胃溃疡作用,其中石油醚萃取部位活性最强。

Objective： To screen the effective fractions of Sanjiu Weitai prescription for anti-gastric ulcer activity.Method： The SD rats were divided into eight groups,including normal control group,model group,positive control（cimetidine and Sanjiu Weitai granules） groups,petroleum ether extract group,ethyl acetate extract group,n-butyl alcohol extraction group and water extraction group,with different dose lavage for seven days.Rat experimental gastric ulcer model was induced by intragastric administration of ethanol.Gastric ulcer inhibition rate,pepsin activity,malondialdehyde（MDA） content and superoxide dismutase（SOD） activity in serum were determined to evaluate anti-gastric ulcer activities of different fractions extracted by petroleum ether,ethyl acetate,n-butanol and water extraction parts.Result： All of the extracts of Sanjiu Weitai prescription could significantly inhibit gastric mucosa damage in rats induced by ethanol（P ＆lt; 0.05）.The gastric ulcer inhibition rate of petroleum ether extract was 89.15%,higher than those of Sanjiu Weitai capsules group（55.31%） and cimetidine group（59.90%）.All of the extracts could significantly reduce pepsin activity（P ＆lt; 0.05） and increase activity of SOD in serum（P ＆lt; 0.05）.Conclusion： All of the various polarity fractions of Sanjiu Weitai prescription can significantly inhibit gastric ulcer.The petroleum ether fraction exhibits the best anti-gastric ulcer activity.