柴胡加龙骨牡蛎汤对创伤后应激障碍的疗效及机制研究

Efficacy and Mechanism of Chaihu Jia Longgu Muli Tang on Posttraumatic Stress Disorder

目的:柴胡加龙骨牡蛎汤(TJ-12)对创伤后应激障碍的疗效及机制。方法:应用ICR小鼠与EL小鼠建立社会失败应激模型,随机分为5组,即模型组及正常对照组、模型+柴胡加龙骨牡蛎汤高、中、低剂量组(1,0.5,0.1 g.kg-1),ig给药,每天1次,连续给药4周。观察条件性恐惧行为,同时用实时RT-PCR法测小鼠海马糖皮质激素受体(GR)及脑神经源性营养因子(BDNF)的基因表达。结果:与正常对照组比较,模型组小鼠出现"僵直"时间百分比延长、海马基因GR与BDNF表达增加,有非常显著性差异。与模型组比较,TJ-12治疗组不仅可以缩短"僵直"时间百分比,且能降低海马BDNF基因的表达,有显著性差异。结论:TJ-12有效治疗创伤后应激障碍可能与调节海马BDNF基因相关。

Objective： To investigate he efficacy and the mechanism of Chaihu Jia Longgu Muli Tang（TJ-12） on posttraumatic stress disorder.Method： ICR mice and EL mice were used to establish the social defeat stress（SS） model.The animals were divided into five groups： TJ-12 high,middle,low dose groups（orally given for 4 weeks,1,0.5,0.1 g.kg-1.d-1）,SS group and control group.Contextual fear conditioning test（CFC） was evaluated,and mRNA expression of glicocorticoid receptor（GR） and brain-derived neurotrophic factor（BDNF） in the hippocampus of the animals were measured by real time RT-PCR.Result： In SS group the freezing pecent scores of CFC test were increased and the mRNA expression level of GR and BDNF in hippocampus of SS mice was also significantly higher than the controls;Compared with the SS group,freezing percent of CFC test and mRNA expression of BDNF in hippocampus were decreased in TJ-12 high,middle,low dose groups.Conclusion： TJ-12 may be effective for treating the posttraumatic stress disorder by altering the gene expression of BDNF in hippocampus.