山麦胶囊对肾性高血压大鼠血压的影响

Effect of Shanmai Capsules on Blood Pressure in Renovascular Hypertensive Rats

目的:观察山麦胶囊对用"两肾一夹"法制备的肾性高血压大鼠血压和心肌肥大模型的影响。方法:采用"两肾一夹"法制备肾性高血压大鼠模型,并用无创血压测量系统筛选出造模成功的大鼠,随机分为模型对照组(生理盐水,NS)、山麦胶囊高、中、低剂量组(1.8,0.9,0.45 g.kg-1)及卡托普利组(7 mg.kg-1),另设空白对照组(NS),连续ig给药4周。于造模前、造模后和给药4周末测量大鼠血压,并于4周末采集大鼠血清和心脏组织标本,用比色法测定血清一氧化氮(NO)含量,酶联免疫法测定血管紧张素Ⅱ(AngⅡ)和内皮素(ET)含量,用精密天平称量全心质量(HW)和左心室质量(LVW),应用病理学方法测定心肌纤维直径(MD)。结果:山麦胶囊高剂量能显著降低肾性高血压大鼠的血压(P<0.01)和血清AngⅡ(P<0.01)、ET(P<0.01)的含量及MD(P<0.01),同时增加血清NO含量(P<0.05)。结论:山麦胶囊对肾性高血压大鼠有降压作用,可抑制心肌肥大的形成。

Objective： To abserve the effects of Shanmai capsules on blood pressure and myocardial hypertrophy in renovascular hypertensive rats induced by ‘two-kidney one-clip’ method.Method： Renovascular hypertensive models were established in rats by ‘two-kidney one-clip’method,then the rat models successfully established were chosen by noninvasive blood pressure measurement system and randomly divided into model group（normol saline,NS）,the high,middle,low dose group of shanmai capsules（1.8,0.9,0.45 g.kg-1,respectively）,captopril group（7 mg.kg-1） and normal control group（NS）,were all orally for 4 weeks.The blood pressure of rats were measured before,and after modeling as well as at the last day of drug delivery.Serum and heart specimens of rats were collected at the end of the 4 week to determined the levels of nitric oxide（NO）,angiotensin Ⅱ（Ang Ⅱ） and endothelin（ET）.The heart weight（HW） and left ventricular weight（LVW） were measured by precision balance.Remodeling of myocardial fiber diameter（MD） was detected by pathologically method.Result： Shanmai capsules at high dose could reduce the blood pressure（P ＆lt; 0.01）,MD（P ＆lt;0.01） and the levels of Ang Ⅱand ET in serum（P ＆lt; 0.01） obviously,meanwhile,increased NO（P ＆lt; 0.05）.Conclusion： Shanmai capsules could lower the blood pressure of renovascular hypertensive rats,inhibit the formation of myocardial hypertrophy.