葛根芩连汤整方剂量变化治疗糖尿病大鼠的量效关系研究

Study of Gegen Qinlian Decoction on Type 2 Diabetes in Rats and the Dose-effect Relationship

目的:探讨葛根芩连汤整方剂量变化治疗2型糖尿病的量效关系,获得基于药效学的经方"剂量阈"。方法:SD大鼠给予高脂饲料喂养4周后按30 mg.kg-1尾iv新鲜配制的STZ(链脲佐菌素)诱导2型糖尿病大鼠模型,正常对照组注射同体积的柠檬酸盐缓冲液。实验分组:正常组、糖尿病模型组、葛根芩连汤整方剂量变化的9个剂量组(分别为生药1.65,4.95,8.25,11.55,14.85,18.15,21.45,24.75,28.05 g.kg-1,)、阳性药物对照组(二甲双胍200 mg.kg-1),取血检测血糖、胰岛素水平、胰岛素抵抗指数(IR)、糖化血清蛋白(GSP)、糖化血红蛋白(HbA1c)等指标。结果:给药10周后,葛根芩连汤明显降低2型糖尿病大鼠血清中的血糖水平与IR(P<0.05,P<0.01)、降低糖化血清蛋白、糖化血红蛋白水平(P<0.05,P<0.01),指标与葛根芩连汤剂量存在着一定的量效关系,在一定剂量范围内,随着葛根芩连汤整方剂量的增大,药效增强,其"剂量阈"为27.01～1.09 g.kg-1。结论:葛根芩连汤整方剂量变化治疗2型糖尿病具有良好的量-效特征。

Objective： To investigate the effects of Gegen Qinlian decoction on type 2 diabetes in rats and the dose-effect relationship,to obtain the dosage threshold of the classical prescription.Method： SD rats were randomly assigned into normal control group and diabetic model group,which was established by injection small dose of streptozotosin（STZ）（30 mg.kg-1） after feeding a diet with high glucose and lipids for 4 weeks.Diabetic rats were randomly assigned into model group,dimethylbiguanide group（200 mg.kg-1） and different dose of Gegen Qinlian decoction groups（1.65,4.95,8.25,11.55,14.85,18.15,21.45,24.75,28.05 g.kg-1 accordingly）.The serum glucose,insulin,insulin resistance（IR）,glycosylated hemoglobin（HbA1c）,glycosylated serum protein（GSP） were determined.Result： Compared with model group,Gegen Qinlian decoction could decrease serum glucose,insulin,IR,HbA1c and GSP level（P ＆lt; 0.05,P ＆lt; 0.01）.As dose of Gegen Qinlian decoction increased,the drug action was more obviously in the specified dose range.The dosage threshold of Gegen Qinlian decoction on diabetes in rats was 27.01-1.09 g.kg-1.Conclusion： The effect of Gegen Qinlian decoction on type 2 diabetes in rats shows fine dose-effect relationship.