三七总皂苷肠溶微丸和三七总皂苷对家兔动脉粥样硬化预防作用比较

Comparison between Panax Notoginsenoside Enteric-coated Pellets and Panax Notoginsenoside in Preventive Effect of Atherosclerosis in Rabbits

目的:比较三七总皂苷(panax notoginsenoside,PNS)肠溶微丸和三七总皂苷对家兔实验性动脉粥样硬化的预防作用。方法:50只家兔随机分为空白对照组、动脉粥样硬化(atherosclerosis,AS)模型组、PNS原料药组、注射用血栓通(冻干)组和PNS肠溶微丸组,耳缘静脉注射牛血清白蛋白250 mg.kg-1后(空白对照组除外),分别喂食普通饲料+生理盐水、高脂饲料+生理盐水、高脂饲料+PNS原料药(30 mg.kg-1.d-1,ig)、高脂饲料+血栓通(15 mg.kg-1.d-1,肌肉注射)和高脂饲料+PNS肠溶微丸(30 mg.kg-1.d-1,ig)。于12周末耳缘静脉取血测定血脂含量,摘取主动脉观察病理学改变。结果:给药12周后,PNS原料药组、注射用血栓通(冻干)组和PNS肠溶微丸组能显著降低家兔总胆固醇(T-CHO),甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平(P<0.01),升高高密度脂蛋白胆固醇(HDL-C)水平(P<0.01);注射用血栓通(冻干)组和PNS肠溶微丸组降低T-CHO,TG和LDL-C的效果优于PNS原料药组(P<0.01),升高HDL-C水平优于PNS原料药组(P<0.01);PNS肠溶微丸组降低LDL-C效果优于注射用血栓通(冻干)组(P<0.01)。主动脉病理结果表明:注射用血栓通(冻干)组和PNS肠溶微丸组均能减轻主动脉内膜病变程度(P<0.01),且两者差异没有统计学意义;PNS肠溶微丸组减轻主动脉内膜病变程度优于PNS原料药组(P<0.01)。结论:三七总皂苷肠溶微丸和三七总皂苷原料药均能降低AS模型家兔的血脂水平和减轻主动脉内膜病变程度,且前者预防作用优于后者。

Objective： To investigate the protective and curative effects between panax notoginsenoside（PNS） enteric-coated pellets and PNS on experimental atherosclerosis（AS） in rabbits.Method： Fifty rabbits were randomly divided into five groups： blank control group,AS model group,PNS group,Xueshuangtong injection（lyophilization） and PNS enteric-coated pellets.They were administered normal diet ＋ NS,high fat diet ＋ NS,high fat diet ＋ PNS（30 mg.kg-1.d-1,ig）,high fat diet ＋ Xueshuangtong（lyophilization,15 mg.kg-1.d-1,im） and high fat diet ＋ PNS enteric-coated pellets（30 mg.kg-1.d-1,ig） respectively after injecting bovine serum albumin 250 mg.kg-1 on ear vein except blank control group.At the end of 12th week,the blood Lipid was detected by ELISA and the pathological changes of aortic intima was observed under light microscopes.Result： After 12 weeks of administration,PNS group,Xueshuangtong injection（lyophilization） and PNS Entericcoated pellets could decrease total cholesterol（T-CHO）,triglycerides（TG） and low-density lipoprotein cholesterol（LDL-C） obviously（P ＆lt; 0.01） and increase high density lipoprotein cholesterol（HDL-C）（P ＆lt; 0.01）.The levels of T-CHO,TG and LDL-C in Xueshuangtong injection and PNS enteric-coated pellets group were lower than PNS group group（P ＆lt; 0.01）,and HDL-C was much higher（P ＆lt; 0.01）.The levels of LDL-C in PNS enteric-coated pellets group was much lower than Xueshuangtong injection group（P ＆lt; 0.01）.The morphological observation showed that Xueshuangtong injection and PNS enteric-coated pellets group could alleviate aortic intimal lesions（P ＆lt; 0.01） and there was no difference between them.The effect of PNS enteric-coated pellets was better than PNS group（P ＆lt; 0.01）.Conclusion： Both PNS entetic-coated pellet and PNS can decrease blood lipid and aortic intimal lesions of AS rabbits.The prevention effect of PNS Entetic-coated pellet is better than PNS on AS rabbits.