期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

TEAD1和YAP1对牛干扰素Tau基因表达调控的试验 被引量：1

Regulation of TEAD1 and YAP1 on Bovine IFNT Gene Expression
下载PDF
导出
摘要 为研究TEAD1和YAP1对牛IFNT基因的表达调控,利用PCR分析检测牛胚胎及各脏器中TEAD1和YAP1的表达情况。并通过IFNT荧光素酶报告基因,分析TEAD1和YAP1对牛IFNT基因在JEG3细胞中表达的影响。结果显示,TEAD1和YAP1基因在牛着床前胚胎中及各脏器中都有表达。荧光素酶分析表明,在IFNT基因5′端上游区域逐渐切除或者点突变的情况下,YAP1对IFNT基因的表达都有显著的上调作用;TEAD1对IFNT基因表达的影响不显著;TEAD1和YAP1共同作用时,协同效果不显著。 In order to study the transcriptional regulation of TEAD1 and YAP1 on bovine IFNT gene expression, PCR analysis was performed to detect the expression of TEAD family and YAP1 in bovine concepus and organs. Through the IFNT Luciferase reporter gene analysis, effect of TEAD1 and YAP1 on IFNT gene expression was studied in JEG3 cells. The result indicated that TEAD1 and YAP 1 gene could be expressed commonly in bovine concepus and organs. YAP1 significantly up-regulated the IFNT gene expression （P〈0.05）, and 5＇-deletion could not impair this up-regulation; TEAD1 had no significant effect on IFNT gene expression~ There was no significant synergistic effect between TEAD1 and YAP1.
作者 张金凤 侯振中
机构地区 东北农业大学动物医学学院
出处 《中国兽医杂志》 CAS 北大核心 2013年第5期26-29,共4页 Chinese Journal of Veterinary Medicine
关键词 牛 TEAD1 YAP1 IFNT Bovine TEAD1 Yap1 IFNT
分类号 S823 [农业科学—畜牧学]
  • 相关文献

参考文献12

  • 1Moor R M and Rowson L E A. Influence of the embryo and u- terus on luteal function in the sheep[J]. Nature, 1964,201: 522-523.
  • 2Imakawa K, Anthony R V, Kazemi M, et al. Interferon-like sequence of ovine trophoblast protein secreted by embryonic tropheetoderm[J]. Nature, 1987,2 330 (6146) : 377-379.
  • 3Stark G R, Kerr I M, Williams B R, etal. How cells respond to interferons[J]. Annu Rev Bioehem, 1998, 67:227-264.
  • 4Spencer T E, Johnson G A, Bazer F W, et al. Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, intererons and endogenous retroviruses [J]. Reprod Fertil Dev, 2007,19(1) :65-78.
  • 5Ideta A, Urakawa M, Aoyagi Y, et al. Early development in utero of bovine nuclear transfer embryos using early G1 and GO phase cells[J]. Cloning Stem Cells, 2007, 9:571-580.
  • 6Sakurai T, Sakamoto A, Muroi Y, etal. Induction of endoge- nous interferon tau gene transcription by CDX2 and high acety- lation in bovine nontrophoblast cells[J]. Biol Reprod, 2009, 80(6) 1223-1231.
  • 7Ezashi T, Ealy A D, Ostrowski M C, et al. Control of inter- feron-tau gene expression by Ets-2 [J]. Proc Natl Acad Sci USA, 1998, 7 95(14):7882-7887.
  • 8Yamaguchi H, Ikeda Y, Taylor A, et al. Effects of PMA and transcription factors on ovine interferon-tau transactivation in various cell lines[J]. Endocr J, 1999, 46 (3) : 383-388.
  • 9Imakawa K, Kim M S, Matsuda-Minehata F, et al. Regula- tion of the ovine interferon-tau gene by a blastocyst-specific transcription factor, Cdx2[J]. Mol Reprod Dev, 2006, 73 (5) ：559-567.
  • 10Bai H, Sakurai T, Kim M S, et al. Involvement of GATA transcription factors in the regulation of endogenous bovine in- terferon-tau gene transcription[J]. Mol Reprod Dev, 2009, 76 (12) : 1143-1152.

同被引文献9

  • 1Bill A,Hall M L,Borawski J,et al.Small molecule-facilitated degradation of ANO1 protein:a new targeting approach for anticancer therapeutics[J].J Biol Chem,2014,289(16):11029-41.
  • 2Kornreich B G.The.patch clamp technique:principles and technical considerations[J].J Vet Cardiol,2007,9(1):25-37.
  • 3Pan Y,Leifert A,Graf M,et al.High-sensitivity real-time analysis of nanoparticle toicity in green fluorescent protein-expressing zebrafish[J].Small,2013,9(6):863-869.
  • 4Galietta L J,Haggie P M,Verkman A S.Green fuorescent protein-based halide indicators with improved chloride and iodide affinitiestJ].FEBS Lett,2001,499(3):220-224.
  • 5George Patterson,Rich N.Day,David Piston.Fluorescent protein spectra[J].Cell Science,2001,11(5):837-838.
  • 6Hong L,Xie Z Z,Dn YH,et al.Alteration of volume-regulated chloride channel during macrophage-derived foam cell formation in atherosclerosis[J].Atherosclerosis,2011,216(1):59-66.
  • 7Terashima H,Picollo A,Accardi A.Purified TMEM16A is sufficient to form Ca~(2+)-activated C1-channels[J].Proc Natl Acad Sci U S A,2013,110(48):19354-19359.
  • 8Filippin L,Abad M C,Gastaldello S,et al.Improved strategies for the delivery of GFP-based Ca~(2+)sensors into the mitochondrial matrix[J].Cell Calcium,2005,37(2):129-136.
  • 9辛文文,王景林.基于荧光检测的新型细胞传感器[J].生物化学与生物物理进展,2011,38(2):185-190. 被引量：2

引证文献1

中国兽医杂志
2013年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部