硫酸黏菌素对原代背根神经元细胞的毒性作用

Study on the cytotoxicity of colistin on dorsal root ganglion neurons in vitro

建立了新生小鼠原代背根节(Dorsal root ganglion,DRG)神经元细胞原代培养的方法,作为评价硫酸黏菌素毒性的体外研究模型。在此模型基础上,使用不同浓度的硫酸黏菌素作用细胞24h后,应用MTT方法观察细胞毒性效应并测定细胞半数抑制浓度(IC50)。结果表明,不同浓度硫酸黏菌素处理细胞后,高剂量组DRG神经元细胞出现明显病变,硫酸黏菌素浓度低于10μg/mL时,对DRG神经元细胞存活率无明显影响,硫酸黏菌素浓度达到10μg/mL以上时,细胞存活率降低,出现细胞毒性,且毒性作用强度随药物浓度增加而增强;试验测得硫酸黏菌素对DRG神经元的半数抑制浓度(IC50)为222.7μg/mL。

To evaluate the cytotoxicity of colistin, the cells culture system of Dorsal root ganglion （DRG） neurons form 3-day mice were established. The MTT assay was applied to measure the cytotoxicity and IC50 after different concentration of colistin treatment of DRG cells for 24 h. Results showed the DRG cells of high concentration group were damaged. Low concentrations （≤10 μg/mL） colistin have no signif- icant toxicity on DRG cells, while in the high concentration, colistin（≥10μg/mL） have significant toxici- ty and the cytotoxicity increased with the increase of concentrations. The ICs0 was 222.7 μg/mL after dif- ferent concentrations of colistin treatment of DRG cells for 24h.