铜负荷大鼠脑组织Cu、Zn、Fe分布及其对神经元细胞损伤的机制

The distribution of trace elements in brain tissue of copper load rats and its mechanism in neuronal cell injury

目的观察铜负荷大鼠不同脑区脑组织Cu、Zn、Fe分布,并探讨其对神经元细胞损伤的机制。方法选择雄性Wistar大鼠50只,随机均分模型组和对照组,模型组给予含硫酸铜1 g/kg的粉状饲料和0.185%硫酸铜水,对照组正常喂饲。第12周结束时,取血、脑组织标本,检测血和不同脑区Cu、Zn、Fe及Cu/Zn-SOD、MDA含量。结果模型组各脑区脑组织Cu含量均较对照组明显升高(P均<0.05),Zn、Fe含量无明显变化;血清、大脑皮层及基底节区Cu/Zn-SOD活力均较对照组减低(P均<0.05),MDA含量均较对照组明显升高(P均<0.05)。结论Cu负荷大鼠脑组织Cu含量明显增高,其对神经元细胞的损伤可能与Cu/Zn-SOD活力减低、MDA含量升高有关。

Objective To observe the distribution of the metal trace elements including copper （Cu）, zinc （Zn） and ferrum （Fe） in different brain regions of brain tissues of copper load rats, and to provide the basis for clarifying the mecha- nism of neuronal cell injury due to the excessive Cu. Methods A total of 50 male Wistar rats aged 3 months were random- ly divided into the model group and the control group. The model group was fed with lg/kg of powdered feed containing copper sulfate and O. 185% copper sulfate water, and the control group was given normal feeding. At the end of 12 weeks, the samples of serum and brain tissues were obtained from these rats. The contents of Cu, Zn, Fe, Cu / Zn-SOD and MDA in blood and different brain regions were detected. Results For the model group, the contents of Cu in each brain re- gions, especially in the basal ganglia, were significantly higher than the control group （all P 〈 O. 05 ）, and no obvious changes in Zn and Fe contens; compared with the control group, the Cu / Zn-SOD activity in the serum, cerebral cortex and basal ganglia were decreased （ all P 〈 O. 05 ）, and the contents of MDA were significantly increased （ all P 〈 0.05 ）. Conclusions The levels of Cu were increased obviously in brain tissues of Cu load rats. For these Cu load rats, Cu / Zn- SOD activity was reduced and the MDA content was increased in serum and brain tissues. These results indicated that ex- cessive Cu can damage the neurons of Cu load rats by increasing the oxidative stress levels of brain tissues.