红腺忍冬组织培养快繁技术(英文)

Tissue culture and rapid propagation of L. hypoglauca

【目的】研究广西山银花当家品种红腺忍冬的组织培养,为红腺忍冬种苗工厂化生产提供技术支持。【方法】以红腺忍冬腋芽为外植体,用0.1%升汞溶液灭菌处理;以MS为基本培养基,探讨不同浓度6-BA、NAA和MET组合对芽诱导、增殖培养和根诱导的影响。【结果】较适宜外植体灭菌的处理为0.1%升汞溶液(加吐温-80)处理7min,外植体污染率为16.0%。春季取材的灭菌效果及腋芽萌发效果最好,冬季最差。较适宜的芽初代诱导培养基为MS+6-BA2.0mg/L,继代培养较适宜的培养基为MS+6-BA2.0mg/L+NAA0.1mg/L,生根培养较适宜的培养基为MS+NAA0.1mg/L+MET3.0mg/L。【结论】通过红腺忍冬腋芽培养,得到可用于生产的组培苗,该技术可应用于红腺忍冬种苗工厂化生产。

[Objective]The tissue culture of L. hypoglauca for dominant Lonicera confusa DC. cultivars in Guangxi was studied to provide technical support for the industrialized production of L. hypoglauca seedlings in Guangxi. [Method]L. hypoglauca axillary buds sterilized by 0.1% HgC12（Mercuric chloride） were used as explants for tissue cul- ture, Different hormone combinations （6-BA, NAA and MET）were added in MS medium to conduct buds induction, multiplication and rooting induction, etc. [ Result ]Comparing all the sterilization treatments, the most suitable one was that dipping shoots in 0.1% HgC12 solution with tweeu-80 for 7 rain, and the contamination rate was 16.0%. The ex- plants sampled in spring had the lowest pollution rate and best axillary bud induction, while the winter samples did the worst. As a result, more suitable culture medium for buds induction was MS＋6-BA 2.0 rag/L, and multiplication cul- ture of axillary buds was found as MS ＋6-BA 2.0 mg/L＋NAA 0.1 mg/L. MS medium with NAA 0.1 mg/L and MET 3.0 mg/L was much more effective for rooting culture. [Conclusion]This technology of getting seedlings by the way of tissue culture of L. hypoglauca may be widely used in L. hypoglauca industrialized breedings ,and large-scale cultivation in Guangxi.