杉木茎段不定芽诱导及植株再生条件筛选

Screening conditions of adventitious bud induction and plantlet regeneration for Cunninghamia lanceolata

【目的】探索广西杉木快繁技术,为杉木组织培养和种苗供应提供参考依据。【方法】以杉木优良树种基部枝条为试验材料,进行茎段外植体消毒、不定芽诱导及植株再生研究。【结果】以75%酒精处理30s+0.1%升汞消毒6min的效果较理想,污染率为30%;初代培养基为1/2MS+NAA0.2mg/L+6-BA0.6mg/L,第8d即有芽萌动,诱导率可达47%;继代培养基1/2MS+IBA0.3mg/L+6-BA0.4mg/L中加入蔗糖30g/L,25d腋芽增殖倍数可达3.4倍;1/4MS+IBA0.15mg/L+NAA0.075mg/L对杉木生根诱导效果较好,生根率为52%。【结论】,杉木外植体用75%酒精和0.1%升汞消毒6min效果较理想,适当减少培养基中矿质元素有利于杉木外殖体芽的诱导和试管苗的增殖生长,NAA可促进试管苗生根。

[Objective]Conditions of the adventitious bud induction and plant regeneration for Cunninghamia lanceolata were screened and rapid propagation techniques for Chinese fir in Guangxi was explored to provide references for supply and tissue culture of Chinese fir seedlings. [Method]Using base branches of superior Chinese fir species as testing materials, stem explant disinfection, adventitious bud induction and plant regeneration were the focal points in the current research. [Result] （1）The best disinfection method was treating stem explants with 75% ethanol for 30 s and 0.1% mercuric chloride for 6 min, which showed 30% contamination rate; （2）In the initial medium （1/2MS ＋ NAA 0.2 mg/L ＋ 6-BA 0.6 mg/L）, the buds germinat- ed on the 8 th day with the induction rate of 47%; （3）In the optimum subculture medium （1/2MS ＋ IBA 0.3 mg/L ＋ 6-BA 0.6 mg/L ＋ white sugar 30 g/L）, axillary buds were multiplied by 3.4 times on the 25th day; （4）The induction effect was the best with the rooting rate of 52% in the rooting medium （1/4MS ＋ IBA 0.15 mg/L ＋ NAA 0.075 rag/L）. [Conclusion]It was beneficial to the adventitious bud induction for Chinese fir with the initial medium （1/2MS ＋ NAA 0.2 mg/L ＋ 6-BA 0.6 mg/L） and the optimum sub- culture medium （ 1/2MS ＋ IBA 0.3 mg/L ＋ 6-BA 0.6 mg/L ＋ white sugar 30 g/L）. NAA was conducive to rooting in vitro , and rooting induction effect was the best in the rooting medium of 1/4MS ＋ IBA 0.15 mg/L ＋ NAA 0.075 mg/L.