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穿透支原体P35蛋白细胞粘附活性研究 被引量：1

Studies on Cyto-adherence of the Mycoplasma Penetrans P35
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摘要 目的研究穿透支原体(Mpe)P35蛋白的细胞粘附活性。方法 IPTG诱导pQE31/p35原核表达载体在E.coli中表达,用Ni-NTA Spin亲和纯化,Western blot鉴定表达产物。通过间接免疫荧光试验(IFA)、竞争抑制试验研究rP35对HeLa细胞的粘附活性。结果 pQE31/p35在E.coli中表达出分子量约35 kDa的蛋白质,Western blot鉴定其为特异性rP35蛋白。IFA发现rP35对HeLa细胞无明显的粘附活性;竞争抑制试验表明rP35不能抑制Mpe粘附HeLa细胞。结论 P35可能不是Mpe的主要粘附分子。 Objective To study the adherence of M.penetrans 35kDa Lipid associated membrane protein（P35）.Methods pQE31/p35,a prokaryotic expression recombinant,was constructed.Expression of recombinant P35 protein（rP35） was induced by IPTG in E.coli.rP35 purified with Ni-NTA Spin Kit was analyzed by Western blot.Indirect immunofluorescence assay（IFA） and competitive inhibition test were used to analyze the adherence of rP35 to HeLa cells.Results rP35 fusion protein with a calculated molecular mass of 35kDa was expressed in E.coli.IFA showed that rP35 could not adhere to HeLa cells.The competitive inhibition test also showed that rP35 was not able to inhibit M.penetrans adhering to HeLa cells.Conclusion Probably P35 is not the main adhesin of M.penetrans.
作者 朱翠明 余敏君 曾焱华 游晓星 吴移谋
机构地区 南华大学微生物学与免疫学教研室
出处 《中南医学科学杂志》 CAS 2013年第3期217-220,共4页 Medical Science Journal of Central South China
基金 国家自然科学基金(81072418/H1005) 湖南省教育厅重点实验室(2012年)项目
关键词 穿透支原体 P35蛋白 细胞粘附 Mycoplasma penetrans P35 adherence
分类号 R375 [医药卫生—病原生物学]
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参考文献15

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二级参考文献16

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同被引文献34

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中南医学科学杂志
2013年 第3期

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