巨大型蒲公英绿原酸的分离纯化及其抑菌实验研究

Purifying and bacteriostatic action of the chlorogenic acid of huge dandelion

通过酒精浸提法提取蒲公英叶中的绿原酸,采用大孔树脂DA-201分离纯化,纸片抑菌法和连续梯度稀释法研究绿原酸的抑菌效果和最小抑菌浓度。结果表明:大孔树脂对绿原酸的静态吸附量为195mg/g,动态吸附量为116.5mg/mL,最佳上样pH值为3,最佳洗脱剂为80%的酒精;通过此方法纯化的蒲公英绿原酸对大肠杆菌、枯草杆菌、根霉和青霉均有良好的抑制作用,其最小抑菌浓度分别为1×10-3、1×10-2、1×10-2、1×10-2mg/mL。

The bacteriostatic action of dandelion chlorogenic acid had been studied by extracting. chlorogenic acid from dandelion using ethanol, purifying using DA-201 and the bacteriostatic action and the MIC were tested by slip method and continuous gradient dilution method. The results showed that： static adsorption capacity of DA-201 was 195 mg/g, dynamic adsorption capacity was 116.5 mg/mL, the best pH was 3, elute concentration was 80% ethanol. The purifying chlorogenic acid by this method had strong bacteriostatic action on Rhizopus, Escherichia coli, Penicillium and Bacillus subitilis. The MIC of chlorogenic acid distinguish for： 1×10-3, 1×10-2, 1×10-2, 1×10-2 mg/mL.