急性髓细胞白血病患者血清中整合素αVβ3、VEGF的表达水平及临床意义

Expression of integrinαVβ3 and VEGF in acute myeloid leukemia and their clinical significance

目的:探讨整合素αVβ3和血管内皮生长因子(VEGF)在急性髓细胞白血病(AML)中的表达及临床意义。方法:应用酶联免疫吸附试验(ELISA)测定32例初发未治AML患者,经治后23例完全缓解,8例复发、9例未缓解AML患者血清中整合素αVβ3与VEGF的蛋白表达水平,并与20例正常对照组比较。结果:初发未治组、未缓解组、复发组患者血清整合素αVβ3和VEGF的含量均明显高于正常对照组和缓解组(P<0.05);初发未治组、未缓解组、复发组患者差异无统计学差异(P>0.05),缓解组患者正常对照组比较,差异无统计学意义(P>0.05)。二者的表达呈正相关。结论:整合素αVβ3和VEGF可能在AML发生、发展及进展过程中起重大作用,其机制可能通过VEGF上调整合素αVβ3促进血管生成,因而通过阻抗二者在血管增生信号通路中的作用而成为白血病治疗的靶点。

Objective： To study the expression of integrinαVβ3 and vascular endothelial growth factor （VEGF） in acute myeloid leukemia （AML） and to evaluate their clinical significance. Methods： 32 patients with uncured acute myeloid leukemia were determined by using enzyme- linked immunosorbent assay （ELISA）. After treatment, 23 patients completely remitted （CR）,8 patients relapsed, and 9 unremitted. The serum levels of integrinαVβ3 and VEGF of the all these groups were compared with 20 normal controls. Re- sults： The serum levels of integrinαVβ3 and VEGF in the groups of uncured, unremitted,and relapsed were sig- nificantly higher than those in normal controls and CR patients （P 〈 0. 05）. The level of integrinαVβ3 and VEGF in patients of uncured, unremmitted, and relapsed had no statistical significance （P 〉 0. 05） and com- paring CR patients with normal control group, there was no statistical significance （P 〉 0.05） either. The ex- pression of VEGF was correlated with the expression of integrinαVβ3 （P 〈 0.05）. Conclusion ：The expression of integrinαVβ3 and VEGF proteins increased in AML patients compared to normal ones, which indicated VEGF and integrinαVβ3 might play an important role in the malignant progression of AML. The mechanism that VEGF can regulate the expression of integrinαVβ3 promotes angiogenesis; therefore, through impeding their roles in vascular proliferation signaling pathways to become the targets for leukemia therapy in the future.