紫草素对胶原诱导关节炎MMP-1和TIMP-1表达影响的研究

Study on Effect of Exogenous MMP-1 and TIMP-1 Expressions on Shikonin in Collagen-induced Arthritis

目的:研究紫草素对Ⅱ型胶原诱导的关节炎(CIA)MMP-1、TIMP-1表达的影响。方法:紫草素及对照药物口服给药CIA小鼠,连用30天,用酶联免疫吸附测定试剂盒测定CIA小鼠血清中的MMP-1、TIMP-1;用逆转录聚合酶链反应测定CIA小鼠髌骨及邻近滑膜组织的MMP-1、TIMP-1表达水平。结果:与模型组(775.9 102.12)相比,紫草素组(255.9 103.7)降低了CIA小鼠血清中MMP-1水平(P<0.001),紫草素组(135.3 9.1)与模型组(106.3 7.07)相比,升高了TIMP-1水平(P<0.05),髌骨及邻近滑膜组织的MMP-1mRNA水平与对照组相比显著被抑制(P<0.05);TIMP-1mRNA水平与对照组相比明显升高(P<0.05)。结论:紫草素可能通过抑制MMP-1,提高TIMP-1发挥对CIA小鼠的治疗作用。

Objective ： To investigate the exogenous MMP - 1 and TIMP - 1 expressions on shikonin in murine collagen - in- duced arthritis （CIA）. Methods ： Shikonin and control drug were given to CIA orally for 30 days. The serum levels of MMP - 1 and TIMP - 1 were measured by enzyme - linked immunosorbent assay Kit. Reverse transcription - polymerase chain reaction （ RT - PCR） was employed to determine the mRNA expressions of MMP - 1 and TIMP - 1 in patella with adjacent synovium in CIA mice. Results：The serum levels of MMP - 1 was decreased in mice treated with shikonin（255.9 103.7） on CIA compared with those in control groups（775.9 102.12） （P 〈0. 001 ） ,and the serum level of TIMP - 1 was up-regulated in mice with CIA after treatment with shikonin （ 135.3 9.1 ） compared with those in control groups （ 106.3 7.07） （ P 〈 0. 05 ）, the mRNA level of MMP - 1 was significantly inhibited both in the syuovial tissue and in the articular cartilage in treated groups compared with those in control groups （ P 〈 0. 05 ） , and the mRNA level of TIMP - 1 was up - regulated in mice with CIA after treatment with shikonin compared with those in control groups （ P 〈 0. 05 ）. Conclusion ： Shikonin may exert its efficacy through the inhibition of MMP - 1 and up-regulation of TIMP - 1.