免疫组化和原位杂交技术检测人工感染PRRSV猪体内病毒定位的比较

Detection of PRRSV by Immunohistochemistry and in Situ Hybridization

探讨免疫组化(IHC)和原位杂交(ISH)方法检测石蜡切片中猪繁殖与呼吸综合征病毒(PRRSV)的特异性和敏感性,为PRRSV在猪体内的定位、致病机理研究等提供有效的试验手段。用免疫组化、原位杂交的方法检测人工感染PRRSV猪的组织,结果在人工感染后7d,用免疫组化在肝脏、脾脏、肺脏、肾脏、扁桃体、胸腺、肺门淋巴结、肠检测到阳性信号;用原位杂交在肝脏、脾脏、肺脏、肾脏、扁桃体、胸腺、肺门淋巴结、十二指肠、大脑检测到阳性信号,两组结果差异不大;但在感染后35d,用免疫组化检测只有肺门淋巴结为阳性,而原位杂交检测显示肝脏、脾脏、肺脏、肾脏、扁桃体、胸腺、肺门淋巴结、十二指肠、大脑仍为阳性。

In order to detect PRRSV in tissue sections,and provide the basis for pathogen distribution and pathogenesis,the immunohistochemistry and in situ hybridization were used to detect the same tissues in piglets at different times postinfection.The results showed that positive signals were found from 7 days postinfection in liver,spleen,lung,kidney,tonsil,hilar node,thymus,intestine with immunohistochemistry,and were found in liver,spleen,lung,kidney,tonsil,hilar node,thymus,intestine,cerebrum with in situ hybridization.The results with IHC and ISH were the same at early stage of infection,but after 35 days postinfection,many positives that negative detected by IHC were found by ISH.The results demonstrated that in situ hybridization method is better than the immunohistochemistry method.