血管内皮生长因子和环氧合酶-2在子宫内膜腺癌中表达及其与肌层侵袭和淋巴转移的关系

Expression of COX-2 and VEGF in endometriai adenocarcinoma and its relationship with myometrial invasion and lymphatic metastasis

目的探讨环氧合酶-2（COX-2）和血管内皮生长因子（VECF）在子宫内膜腺癌中表达及其与肌层侵袭和淋巴转移的关系。方法收集濮阳市妇幼保健院医院2002年9月至2012年9月手术切除子宫内膜腺癌标本60例，根据有无深肌层浸润和（或）淋巴转移将子宫内膜腺癌分为两组，应用荧光定量聚合酶链式反应（PCR）、蛋白印迹法测定腺癌组织中COX-2、VEGF的表达水平，以正常子宫内膜组织作为对照组。结果①子宫内膜腺癌组织COX-2与VEGF mRNA表达呈正相关（r=0．7137，P=0．0001）；②对照组中COX-2和VEGFmRNA表达（4．8±1．1、3．4±0．7）均高于低危组（3．8±0．9、2．6±1．0），差异有统计学意义（P〈0．05）；③低危组中COX-2和VEGF蛋白表达（0．13±0．10、0．68±0．16）均低于高危组（0．20±0．12、0．83±0．14），差异有统计学意义（P〈0．05）。结论子宫内膜腺癌组织中COX-2可能上调VEGF的表达水平，促进新生血管和淋巴管生成，从而促进肌层浸润和淋巴转移，为临床预防、治疗及预后判断提供参考。

Objective To investigate the expression of COX-2 and VEGF in endometrial adenocarcinoma and its relationship with myometrial invasion and lymphatic metastasis. Methods According to the presence or absence of myometrial invasion and lymphatic metastasis, 60 endometrial adenocareino- ma cases were divided into two groups, and 30 normal endometrial tissue as control group. The expressions of COX-2 and VEGF in different endometria hissues were examined by RT-PCR and western-blot. Results （1） VEGF mRNA expression was positively correlated with COX-2 in the endometrial adenocarcinoma tissue （ r = 0. 7137, P = 0. 0001 ）. （2）The mRNA levels of COX-2 and VEGF in the control group were higher than that in the low risk group, the difference was statistically significant（ P 〈 0. 05 ）. （3） The protein levels of COX-2 and VEGF in the low-risk group were lower than that in the high-risk group, the difference was statistically significant （ P 〈 0. 05 ）. Conclusions The COX-2 may upregulate the expression of VECF in endometrial adenoearcinoma tissues, and promote ansiogenesis and lymphangiogenesis. Accelerate myometrial invasion and lymphatic metastasis, provide reference of clinical prevention, treatment and prognosis assessment.