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中国环颈雉A-FABP基因cDNA部分序列测定与组织表达分析 被引量:1

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摘要 运用RT-PCR方法从中国环颈雉的总RNA中克隆了脂肪型脂肪酸结合蛋白基因的cDNA部分序列,然后运用Real-time PCR法检测了A-FABP基因在中国环颈雉不同发育阶段、不同组织及吉林黄鸡和AA白羽肉鸡的表达情况。结果显示:中国环颈雉A-FABP基因部分cDNA序列为412 bp,与鸡、鸭的基因序列同源性较高,具有脂质运载蛋白家族结构域特征。中国环颈雉A-FABP基因在150日龄表达量最大,脂肪组织为优势表达组织,不同品种间吉林黄鸡表达量最大。中国环颈雉A-FABP基因的研究,为进一步研究中国环颈雉的生长调控和良种开发与利用提供理论依据。
出处 《畜牧与兽医》 北大核心 2013年第6期50-54,共5页 Animal Husbandry & Veterinary Medicine
基金 国家科技基础条件平台建设项目(2005DKA21102)
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