长期低剂量微波辐照对大鼠血小板的L-精氨酸/NO系统的影响

The research about L-arginine nitric oxide pathway in rat platelets with the Long-time low dose Microwave

目的:研究长期低剂量微波对大鼠血小板左旋精氨酸/一氧化氮(L-arginine nitric oxide pathway,L-Arg/NO)系统的影响,为长期暴露于低剂微波下血小板功能的影响提供实验依据。方法:采用峰值功率为5W/cm2的微波辐照健康成年雄性SD大鼠,1小时/天,连续40天,而后检测辐照后大鼠尾部出血时间、血清NO(nitric oxide)含量、血小板源性NO(platelet-derived nitric oxide,PDNO)含量及血小板一氧化氮合酶(nitric oxide synthase,NOS)活性的变化。结果微波辐照组与对照组相比,尾部出血时间显著缩短(P<0.01),血清NO含量、PDNO含量及血小板NOS活性均有非常显著的降低(P<0.01)。结论:长期低剂量微波辐照后NO的抗血小板聚集作用减弱,血小板活化与血小板L-Arg/NO系统功能紊乱有关。

Objective： To explore the association among L- arginine nitric oxide pathway of platelet and the functional changes of platelet with the Long- time low dose microwave. Methods： Healthy adult male SD rats were exposed to low dose Microwave. （the peak power density of 5W/cm2） for lh/d, it lasted 40 days. Then the tail bleeding time （BT} of rats, platelet- derived NO content （PDNO）, NOS activity in platelets and serum NO level were detected after the irradiation. Results： Comparing with the control group, BT in chronic irradiation group shortened very signifieandy（P 〈 0.01 ）. PDNO, NOS activity in platelets and sertun NO decreased, very significantly（ P 〈 0.01 }. Conclusion： The results of this study suggest that the inactivation effect of NO on platelet was reduced, and the platelet activation related to L- arginine nitric oxide pathway function disorders of platelet in rats exposed to long- time low dose microwave.