2009-2012年广西南宁市公共场所中央空调冷却水

Surveillance Results and Analysis ofLegionella pneumophilain Cooling Water of Central Air-conditioning Systems in Public Places in Nanning, Guangxi,2009-2012

目的了解南宁市冷却水嗜肺军团菌污染情况及基因型特征,为预防和控制军团菌病提供依据。方法 2009年1月-2012年12月,采集南宁市部分公共场所中央空调冷却水进行病原分离和基因鉴定。结果冷却水中嗜肺军团菌阳性率达21.98%,阳性率呈逐年递减趋势;除1-4月外,各月份均有嗜肺军团菌检出;脉冲场凝胶电泳(PFGE)聚类分析结果显示南宁市嗜肺军团菌1型(Lp1)的遗传相似度差异有统计学意义。结论南宁市公共场所中央空调冷却水嗜肺军团菌污染严重,菌型多样,且嗜肺军团菌1型菌株呈现遗传多样性。

Objective To investigate the contamination status of Legionella pneumophila（Lp） in the cooling water of the central air-conditioning systems in the public places in Nanning,to identify the genetic features of the target organism,so as to provide basis for the control and prevention of Legionella disease.Methods The cooling water of the central air-conditioning systems in the public places in Nanning were collected from January,2009 to December,2012 for testing Legionella pneumophila and identifying their genetic features.Results The detection rate of Legionella pneumophila in the cooling water from 2009 to 2012 was 21.98%,and it decreased year by year.Legionella pneumophila was detected in the cooling water each month except from January to April.The pulsed field gel electrophoresis（PFGE） indicated significant difference in the genetic similarity in most of the Lp1 strains.Conclusions Contamination by Legionella pneumophila in the cooling water of the central air-conditioning systems in the public places in Nanning is serious.The Lp1 strains are found to have genetic diversity.