脑外伤患者脑脊液IL-1β、IL-6、IL-8、TNF-α、IL-10水平变化及临床意义

Change of IL-1β,IL-6,IL-8,TNF-α,IL-10 in cerebrospinal fluid after brain injury and its clinical significance

目的探讨脑外伤时不同时期脑脊液IL-1β、IL-6、IL-8、TNF-α、IL-10的水平变化及临床意义。方法选择48例脑外伤患者,其中重型26例、轻型22例;高颅内压25例、低颅内压23例。分别留取伤后12、24、36、72 h的脑脊液标本,采用酶联免疫吸附法检测伤后各时间点脑脊液IL-1β、IL-6、IL-8、TNF-α、IL-10水平。另取查体健康者28例为对照组。结果与对照组相比,脑外伤患者脑脊液中IL-6、IL-1β、TNF-α、IL-8和IL-10水平均显著增高(P均<0.01)。脑外伤重型组脑脊液IL-6、IL-1β、TNF-α和IL-8峰值水平高于与轻型组(P均<0.01);高颅内压组脑脊液中IL-1β峰值水平高于低颅内压组(P<0.01)。结论脑外伤后脑脊液炎性细胞因子水平升高,可作为预测脑损伤严重程度的指标。

Objective To investigate the concentration change of the inflammatory cytokines （ IL-1β、IL-6、IL-8、TNF-α、IL-10） in cerebrospinal fluid at different periods of time after traumatic brain injury. Methods In this study, 48 patients with traumatic brain injury were enrolled, the cerebrospinal fluid samples were collected in 12 h, 24 h, 36 h and 72 b after traumatic brain injury, the concentration of the inflammatory cytokines were detected with enzyme-linked immu- nosorbent assay. Results Compared with that in control group, there were significantly increased concentrations of IL-1β、IL-6、IL-8、TNF-α、IL-10 in patients with traumatic brain injury（ all P 〈0.01 ）. Peak concentrations of IL-1β、IL-6、IL-8、 of eerebrospinal fluid in patients with severe brain injury were significantly higher than those in patients with mild brain injury（ all P 〈 0.01 ）. Peak concentration of IL-1βin patients with high intracranial pressure was higher than that in patients with low intraeranial pressure（P 〈 0.01 ）. Conclusion The levels of IL-1β、IL-6、IL-8、TNF-α and IL-10increase after the traumatic brain injury, which may be one of the probable biomarkers for the severity of brain injury.