极大螺旋藻多糖的分离纯化及化学结构分析

ISOLATION, PURIFICATION AND CHEMICAL STRUCTURE ANALYSIS OF POLYSACCHARIDE FROM SPIRULINA MAXIMA

极大螺旋藻干粉经热水提取、醇析、Sevag法脱蛋白得粗多糖 ,经SephadexG 10 0凝胶柱层析得白色粉末状多糖纯品 (PolysaccharideofSpirulinamaxima ,PSM ) .纸层析、凝胶柱层析、高效液相色谱分析表明为均一组分 .硫酸 咔唑法、硅胶薄层层析和气相色谱分析表明PSM是一种由L 鼠李糖、D 木糖、D 葡萄糖、D 半乳糖、D 阿拉伯糖、D 甘露糖和葡萄糖醛酸组成的酸性杂多糖 ,其摩尔比为 1.94 7∶0 .90 5:1∶1.182∶ 0 .338∶ 0 .34 7∶1.330 .高碘酸氧化和Smith降解表明该多糖中主要含有 1→ 3位和 1→ 6位键合的糖苷键 ,其主链由L 鼠李糖以 1→ 3位键合的糖苷键组成 .红外光谱分析和核磁共振氢谱分析表明其糖苷键键型为α型 .SephadexG 2 0 0凝胶柱层析表明其分子量约为 2 .

Polysaccharide of Spirulina maxima (PSM) was isolated and purified from Spirulina maxima by hot water extraction, alcohol precipitation, Sevag method and gel filtration(Sephadex G 100). Its homogeneity was examined by paper chromatography, Sephadex G 200 gel column chromatography and HPLC. The average molecular weight of PSM is 29 500. Infrared analysis and the 1H spectrum of nuclear magnetic resonance analysis showed that PSM has α glycoside linkage. Its mono saccharide composition was identified by thin layer chromatography and GC. PSM consists of L rhamnose, D arabinose, D xylose, D mannose, D glucose, D galactose and glucuronic acid in the ratio of 1.947:0.338:0.905:1:0.347:1.182:1.330. The results of periodate oxidation and Smith degradation showed that the major linkage of PSM is (1→3) L rhamnose.