鹤顶兰嵌合体分离研究初探

Separation of Phaius tankervilleae Chimera

以秋水仙素诱导获得的鹤顶兰嵌合体无菌苗的假鳞茎、茎尖及除根苗为外植体,分别接种于花宝一号的固体、半固体及固液双层培养基上进行培养,通过诱导类原球茎的方法对嵌合体进行分离。结果表明,鹤顶兰嵌合体分离最佳方法为将除根苗插入花宝一号+100.0 ml/L椰子水+2.0 g/L活性炭+20.0 g/L蔗糖+2.0 mg/LBA+0.2 mg/LNAA的半固体培养基上,诱导出PLB进而分化成苗,分离得到纯合四倍体。

In order to obtain pure teraploid of Phaius tankervilleae, improve polyploidy induction efficiency. Through inducting protocorm like body for separation of chimera, the research used the pseudobulb, root segment, shoot apex as explants cultured with Hyponex No.l, solid, semi-solid and liquid-solid double-layer medium. The results showed that the best method of inducting protocorm iike body was inserting the resection of the plant root to semi-solid Hyponex No.1 ＋ 100 mYL CW ＋ 2 g/L AC ＋ 20 g/L sucrose ＋ 2.0 mg/L BA ＋ 0.2 mg/LNAA. Two pure teraploid were obtained from this. The method can be used to separate Phaius tankerviUeae chimera.