摘要
以秋水仙素诱导获得的鹤顶兰嵌合体无菌苗的假鳞茎、茎尖及除根苗为外植体,分别接种于花宝一号的固体、半固体及固液双层培养基上进行培养,通过诱导类原球茎的方法对嵌合体进行分离。结果表明,鹤顶兰嵌合体分离最佳方法为将除根苗插入花宝一号+100.0 ml/L椰子水+2.0 g/L活性炭+20.0 g/L蔗糖+2.0 mg/LBA+0.2 mg/LNAA的半固体培养基上,诱导出PLB进而分化成苗,分离得到纯合四倍体。
In order to obtain pure teraploid of Phaius tankervilleae, improve polyploidy induction efficiency. Through inducting protocorm like body for separation of chimera, the research used the pseudobulb, root segment, shoot apex as explants cultured with Hyponex No.l, solid, semi-solid and liquid-solid double-layer medium. The results showed that the best method of inducting protocorm iike body was inserting the resection of the plant root to semi-solid Hyponex No.1 + 100 mYL CW + 2 g/L AC + 20 g/L sucrose + 2.0 mg/L BA + 0.2 mg/LNAA. Two pure teraploid were obtained from this. The method can be used to separate Phaius tankerviUeae chimera.
出处
《热带农业科学》
2013年第5期43-45,共3页
Chinese Journal of Tropical Agriculture
基金
教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(No.lhxm-2012-7)
2013年海南省自然科学基金项目(No.313032)
公益性行业(农业)科研专项经费项目(No.201203071)
三亚市院校专项资金项目(No.YD09036)
关键词
鹤顶兰
嵌合体
组织培养
四倍体
Phaius tankervilleae
chimera
tissue culture
tetraploid