D-核糖产生菌的选育及发酵

Study of Breeding D-ribose-secreting Strains and Its Fermentation

以枯草芽孢杆菌Ｄ－２ 为出发菌株，经紫外诱变，得到一株莽草酸缺陷型突变株Ｄ－２０２，摇瓶发酵Ｄ－核糖产量可达到５２．６ｇ／Ｌ。该菌遗传稳定性良好。通过发酵条件的优化，最高产量可达６５ｇ／Ｌ。经２ｔ发酵罐中试，发酵单位达到５０ｇ／Ｌ。

A transketolase deficient Bacillus subtilis D 202 which could produce 52.6 g/L D ribose in the shaking flask was obtained from Bacillus subtilis D 2 through ultraviolet irradiation. The strain's heredity was stable. 65g/L D ribose was excreted under optimization conditions. The output of D ribose was achieved 50g/L in a 2 ton fermenter.