生物合成二酮哌嗪类化合物的环二肽合酶研究进展

Research Progress of Cyclodipeptides Synthases

环二肽合酶(CDPSs)是新发现的一类与非核糖体肽合成酶(NRPSs)不同的合成非核糖体肽的酶。该类酶以氨酰-tRNA(aa-tRNA)作为底物合成具有广泛生物活性的化合物——二酮哌嗪类化合物,CDPSs的催化机制比较特殊,只能利用20种天然氨基酸形成的氨酰-tRNA作底物,在后修饰酶的作用下形成环二肽类化合物。CDPSs的晶体结构与Ⅰ型氨基酸-tRNA合成酶(aa-tRSs)的催化结构域类似,但催化机制不同。CDPSs蛋白非常小,但与NRPSs一样,能催化完整的环二肽类化合物的合成。

Cyclodipeptides synthases（CDPSs）,a newly defined family of enzymes, distinct from nonribosom-al peptide synthases （NRPSs） and hijack aminoacyl-tRNA （aa-tRNA）as substrate to synthesize diketopipera- zines （DKPs） which are the precursors of a large class of natural products with noteworthy biological activities. The crystallographic architecture of CDPSs is very similar to that of the catalytic domains of class-I aminoacyl- tRNA synthases（aa-tRSs） although the enzymes have different catalytic mechanism. Compared with NRPSs, CDPSs are small size enzymes and utilize the natural amino acids with the help of cyclodipeptide-tailoring to ac- complish the task of synthesing the final cyclodipeptides.