RP-HPLC法测定阿托伐他汀钙片的含量

Determination of Atorvastatin Calcium Tablets by RP-HPLC

用反相高效液相色谱法测定阿托伐他汀钙片的含量。以C18柱（250mm×4．6naA,n，5wn）为色谱柱，乙腈-四氯呋哺-0．05mol／L枸橼酸溶液（26：19：55）为流动相，检测波长244nm，流速1．0mL／min。阿托伐他汀在9．3-185．6μ／mL浓度范围内，峰面积与浓度线性关系良好。平均回收率为99.35％，RSD％为0．45。本方法简便、准确、重现性好，可用于阿托伐他汀钙片的含量测定。

To establish a method for the determination of Atorvastatin Calcium tablets by RP-HPLC. The chromatography was performed on a C18 （250 mm×4.6 mm, 5 μm） column, the mobile phase was acetonitrile - tetrahydrofuran - 0.05 mol/L citric acid solution （26：19：55）, the detection wavelength was 244 nm, and the rate was 1.0 mL/min. The calibration curves were linear within a range of 9.3-185.6 μg/mL （r = 0. 9999） for Atorvastatin. The average recovery was 99.35 % and RSD % was 0.45. This method was simple, accurate and repeatability, and can be used for the quality control of Atorvastatin Calcium tablets.