马鞭草中总黄酮的超声波辅助提取及其抗氧化活性研究

Study on the ultrasonic-assisted extraction and antioxidant activity of total flavonoids from Verbena officinalis L.

采用正交试验设计研究超声波辅助提取马马鞭草中总黄酮的工艺条件,并对马鞭草中黄酮类化合物的抗氧化活进行测定.结果表明,马鞭草中总黄酮的最佳超声提取工艺条件为乙醇溶液体积分数70%、固液比1∶40(g/mL)、超声功率500 W、超声辅助提取温度70℃条件下提取20 min,在此条件下提取率可达2.87%.影响马鞭草中总黄酮提取效果的主次因素为:固液比>超声波功率>提取温度>乙醇体积分数.马鞭草黄酮类化合物具有清除羟自由基、超氧阴离子自由基的作用,其清除效果在一定范围内随着黄酮类化合物质量浓度的增加而增强.

The orthogonal experimental design method was employed to study the ultrasonic -assisted extraction process of total flavonoids from Verbena officinalis L.and their scavenging activities against hydroxyl and superoxide anion free radicals were evaluated.The optimum conditions for extracting total flavonoids from Verbena officinalis L. were found to be： ethanol concentration 70%, solid -to -liquid ratio 1∶40 （g/mL）, ultrasonic power for 500 W, extraction temperature 70 ℃ and length of ultrasonic treatment of 20 min.Under these optimum conditions , the extraction efficiency of total flavonoids reached as high as 2.87%.Extraction temperature had the most important effect on the extraction of total flavnoids from Verbena officinalis L., followed by length of ultrasonic treatment, material -to -liquid ratio and ethanol concentration .The flavonoids extraction from Verbena officinalis L.had marked scavenging effects on both hydroxyl and superoxide anion free radicals in a concentration -dependent fashion.