拮抗菌和药剂对碱蓬病原菌的抑菌试验初报

The preliminary report on antibacterial activity of antagonistic bacteria and farm chemicals to Suaeda pathogens

碱蓬是一种在生态、食用、医疗保健等诸多方面均有巨大开发价值的野生植物资源.从大连地区辽宁碱蓬(Suaeda liaotungensis Kitag)感染红斑病的叶片中,单斑分离出2种病原真菌SK-X1和SK-X2,采用菌落抑制法,以5种细菌和2种药剂对其进行了抑菌活性检测,旨为碱蓬生产栽培中的红斑病防治提供理论依据.经鉴定SK-X1为拟盘多毛孢属(Pestalotiopsis stey)、SK-X2为炭疽菌属(Colletotrichum)真菌;抑菌试验结果表明,巨大芽孢杆菌(Bacillus megatherium)对SK-X1具有较好的抑菌效果,抑菌率为65.3%,抑菌效果相当于80μg/mL的百菌清、150μg/mL硫酸铜.200μg/mL硫酸铜对SK-X1和SK-X2抑菌率分别达到98%和86.1%.

Suaeda glauce is a kind of valuable wild vegetables with good food and medical values, which has beneficial effects on the remediation of soil. In this study,two kinds of fungi, SK-X1 and SK-X2,were separated from the single spot on the red leaf spot leaves of infected Suaeda liaotungen- sis Kitag. In order to provide a theoretical basis to control the red leaf spot in Suaeda cultivation, five kinds of antagonistic bacteria and two kinds of farm chemicals were tested for the antibacterial activi- ty by colony inhibition method. The strain SK-X1 was identified as Pestalotiopsis stey and SK-X2 be- longs to Colletotrichurn. The result shows Bacillus rnegatheriurn has obvious bacteriostatic effect to SK-X1. Its inhibition rate is 65.3% ,which is equal to 80μg/mL chlorothalonil or 150μg/mL copper sul- fate. The inhibition rate of 200 μg/mL copper sulfate to SK-X1 and SK-X2 is 98% and 86.1%, re- spectively.