复合酶提高黄柏水提效率的关键技术研究

Research on Key Technology of Improvment on the Efficiency of Cortex Phellodendri Chinensis Waterextraction by Composite Enzyme

目的探讨提高黄柏水提工艺效率的方法。方法在黄柏水提工艺程序中,加入纤维素酶-果胶酶-半纤维素酶组成的复合酶,按均匀试验设计考察5因素10水平,用SAS8.2统计分析,测定过滤速度及水提液的浸出物变化作指标,考察酶解工艺条件。结果每克黄柏取相当于酶活力1万U的纤维素酶,0.5万U的果胶酶,0.1万U的半纤维素酶,调温度37℃,pH为3.9的酶解条件下得到的黄柏水提液,分离速度提高5.64倍,黄柏碱与小檗碱收率分别提高2.17、1.14倍。结论应用复合酶可提高黄柏水提工艺的生产效率。

Objective： To study and improve the efficiency of the Cortex Phellodendri Chinensis water-extracting process.Methods： The process of Cortex Phellodendri Chinensis water-extraction was added in composite enzyme which comprises of ＂cellulose enzyme＂-＂pectinase＂-＂half of cellulose enzyme＂,with the basis on the uniform experimental design investigating the 5 factors and 10 levels,with the SAS 8.2 statistical analysis.The filtration speed and water extraction fluid extract change index was examined enzyme solution process conditions.Results： Each 1g cortex phellodendri taking the enzyme activity was equivalent to 10000 U the cellulose enzyme,5000 U pectinase,1000 U half cellulose enzyme.The temperature was 37 ℃,pH 3.9 of enzyme solution obtained under such condition.The separating speed was increased by 5.64 times,cortex phellodendri alkali and berberine were increased yield 2.17,1.14 times.Conclusion： Application of composite enzyme can improve the Cortex Phellodendri Chinensis water-extracting process production efficiency.