电针对帕金森病模型大鼠海马CA1区nNOS表达的影响

Effect of electroacupuncture on nNOS expression at hippocampal CA1 in the model rat of Parkinson’s disease

目的:观察不同频率电针对帕金森病(PD)模型大鼠海马CA1区神经元型一氧化氮合酶(nNOS)表达的影响。方法:将24只SD大鼠随机分为4组:正常对照组、模型组、PD模型低频电针组和高频电针组。采用右侧纹状体内注射6-羟基多巴胺(6-OHDA)制备PD模型,取合谷和太冲穴,分别给予低频(2 Hz)和高频(100 Hz)电针治疗。免疫组织化学方法观察海马CA1区nNOS表达。结果:与正常对照组相比,PD模型大鼠海马CA1区nNOS表达增加,高频电针可降低其nNOS表达,低频电针对其没有影响。结论:高频电针治疗PD的机制之一可能是通过降低PD模型大鼠海马CA1区nNOS表达。

Objective： To observe the variation of neuronal nitric oxide synthase （nNOS） expression at the hippoeampal CA1 region in model rats with Parkinson＇s disease （PD） by electroaeupuncture （EA） stimulation at different frequency. Methods：Twenty four Sprague-Dawley rats were equally randomized into groups of normal controls, models, low-frequency EA and high- frequency EA treatment. PD models of rats were developed by injection of 6- hydroxyl-dopamine （6-OHDA） into the right striatum, and undergone electrotherapy with EA at low-frequency （2 Hz） and high-frequency （ 100 Hz） via acupuncture point of Hegu or Taichong. immuno- histochemieal method was used to observe the changes of nNOS expression at hippocampal CAl. Results： Increased nNOS expression was found at hippocampal CA1 region in PD model rats as compared with the controls. High-frequency EA resulted in decrease of nNOS expression, yet the stimulation by low-frequency EA produced no effect. Conclusion： Effective stimulation via high-frequency EA for PD may be associated with one of the potential mechanisms of decreased nNOS expression at hippocampal CA1 region.