摘要
利用反向PCR法,缺失人IL-8cDNA内部bp序列,构建得到IL-8突变内标分子.用 已知量的该内标分子,通过竞争性RT-PCR法、检测支气管哮喘患者外周血IL-8基因的表达情 况,发现患者IL-8mRNA的表达水平明显高于正常人对照组.这一结果初步表明,利用竞争 性RT-PCR技术对支气管哮喘息者进行早期基因诊断,具有一定的可行性。
Competitive reverse tracscription polymerase chain reaction (competitive RT-PCR) is the most efficient method for accurately measuring mRNA level in samples of total RNA. We constructed mutant internal standards, which deleted 95 base pair nucleotides in internal coding sequence of interleukin-8 (IL-8) by inverse PCR technique. The expression level of IL-8 mRNA in asthmatic patients was determined by RT-PCR with this competitive internal standard. The experiment results showed that the mRNA level of IL-8 in asthmatic patients were markedly higher than that in normal people. This suggested it is possible for asthma early diagnoss using hte IL-8 internal standards.
出处
《中山大学学报(自然科学版)》
CAS
CSCD
北大核心
2000年第2期92-96,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni