MG63细胞验证无镍不锈钢生物相容性的研究

Evaluation on Biocompatibility of Nickel-free Stainless Steel by MG63 Cell Lines

目的:利用MG63细胞系来检测新型生物材料无镍不锈钢的生物相容性。同时初步探讨无镍不锈钢应用于口腔临床的可行性。方法:将MG63细胞接种于3种金属(无镍不锈钢、317L不锈钢和CoCrMo合金)表面,连续培养5 d,比较在3种金属表面的细胞黏附率、细胞增殖率、碱性磷酸酶活性。结果:初次接种12 h,3种金属表面的细胞黏附率差异有显著性,其中,无镍不锈钢高于317L不锈钢和CoCrMo合金。在第3天和第5天时,无镍不锈钢的碱性磷酸酶的活性高于另外2种金属;无镍不锈钢的细胞增殖率高于另外2种金属。结论:无镍不锈钢的生物相容性要好于317L不锈钢和CoCrMo合金。

Objective： To detect new biocompatibility of nickel-free stainless steel using MG63 cell lines. Meanwhile in order to discuss the feasibility of high nitrogen steels used in oral clinic. Methods： MG63 cells were seeded in three groups of the metal sur- face （ nickel-free stainless steel, steel 317L and CoCrMo alloy）, cultured for 5 days. Cell adhesion rate, the rate of cell proliferation, alkaline phosphatase activity were compared. Results： At 12 h, cells adherent rate in nickel-free stainless stell was higher thon that in other two metals. At the third and fifth day, alkaline phosphatase activity in nickel-free stainless steel was higher than that in other two metals. MTI＇ assay of cells in the third and fifth day, the cell proliferation in nickel-free stainless steel was higher than that in oth- er two metals. Conclusion： Biocomoatibilitv of nickel-free stainless steel is better than 317L and CoCrMo alloy.