蒙药材通经草中盐酸小檗碱的提取与含量的测定

Extraction and Determination of Hydrochloric Acid Berberine from Aleuritopteris Argentea(Gmel)Fee

以浓盐酸-甲醇(1∶100)为提取溶剂,采用先浸泡,后水浴加热、超声和先水浴加热、超声后浸泡两种提取方法从通经草中提取生物碱,用分光光度法在波长为426nm处分别测定盐酸小檗碱的含量,回归方程为Y=0.00393+1.28929X(mg/10mL),r=0.99992.通经草中盐酸小檗碱的含量在0.099～0.796 mg/10mL范围内与吸光度呈线性关系.样品盐酸小檗碱回收率分别为93.08%～108.58%,93.08%～100.84%.该结论可为通经草药材的进一步开发利用提供理论依据.

This paper discussed extraction of Hydrochloric acid berberine from Aleuritopteris argentea（Gmel）Fee by meas of two different extraction methods,use hydrochloride acid-methanol （1：100） as solvent extraction. One method is immersed Aleuritopteris argentea （Gmel） Fee firstly and then water-bolt, ultrasonic extraction. The other is water-bolt, ultrasonic Aleuritopteris argentea（Gmel）Fee firstly and then immersed. The content of Hydrochloric acid berberine was determined by spectrophotometry at the wavelength of 426 nm respectively. Regression equation is Y = 0.00393 ＋ 1.28928X.（mg/10mL）, r = 0.99992, Hydrochloric acid berberine within the concentration of 0.099 - 0.796 （mg/mL） had linear relation with absorbance. The rates of recoveries are 93.08% - 108.58% and 93.08% - 100.84%. The research could provide a theoretical foundation for further development and utilization, of Aleuritopteris argentea （Gmel） Fee.