毛大丁草根水煎剂对受试动物的镇咳、祛痰、平喘作用

Effect of Gerbera piloselloides Decoction on Preventing Cough,Eliminating Phlegm and Relieving Asthma on Mice

目的:观察毛大丁草根水煎剂对受试动物的镇咳、祛痰、平喘作用。方法:采用氨水诱咳法分别观察毛大丁草根水煎剂对小鼠咳嗽次数及咳嗽潜伏期的影响;酚红排泌法检测毛大丁草根水煎剂对小鼠呼吸道酚红排泌量的影响;离体气管法观察毛大丁草根水煎剂对乙酰胆碱(Ach)、组胺(His)诱导的豚鼠离体气管平滑肌收缩的影响。结果:毛大丁草根水煎剂可减少小鼠咳嗽次数、延长咳嗽潜伏期;可增加小鼠呼吸道酚红排泌量;可降低Ach、His诱导的豚鼠离体气管平滑肌的收缩张力。结论:毛大丁草根水煎剂对受试动物具有镇咳、祛痰、平喘作用。

Objective： To observe the effects of antitussive, expectorant and anti-asthmatic of Gerbera piloselloides （L.） cass root on the experimental animals. Methods： Mouse cough model was induced by ammonia water, the times and incubation period of cough on mice was recorded. The phenol red excretion test was applied to detect the amount of phenol red excreted by respiratory tract, and the guinea pig tracheal in vitro was adopted to record the effect of contractive tension of the isolated tracheal smooth muscle induced by acetylcholine （Ach） and histamine （His）. Results： Data showed that the decoction extracted from the Gerbera piloselloides （L.） cass root could reduce the cough times, prolong the cough incubation period of the mice, increase the amount of phenol red excreted by respiratory tract; and reduce the tension of contraction of isolated tracheal smooth muscle induced by Ach and His. Conclusion： The water decoction from Gerbera piloselloides （L.） cass root has effects of preventing cough, eliminating phlegm and relieving asthma on experimental cough animal.