益肾活血平喘合剂对哮喘大鼠气道TGF β_1表达的影响

Effects of YiShenHuoXue Decoction on TGF β_1 Expression in Asthma Rats

目的:探讨益肾活血平喘合剂对哮喘大鼠气道TGF β1表达的影响。方法:健康雌性SD大鼠60只。随机分为4组,即正常组、模型组、地塞米松阳性对照组、益肾活血平喘合剂组,每组15只大鼠。除正常组外,其余3组均利用哮喘模型。各组于造模后14 d开始每天给药及雾化吸入。造模后28 d处死动物,取同一部位肺叶,包埋,切片,HE染色及免疫组化检测气道TGF β1的表达。结果:益肾活血平喘合剂组气道TGF β1的表达明显少于模型组(P<0.01)及阳性对照组(P<0.05)。结论:益肾活血平喘合剂通过抑制气道TGF β1的表达,减轻哮喘气道炎症及重塑的发生。

Objective： To investigate the effects of Yishenhuoxuepingchuan （YSHXPC） decoction on TGF β1 expression in airway of asthma rats. Methods： Sixty health female SD rats were randomly divided into 4 groups （15 in each）： the normal group （normal rats＋saline）, the model group （athema rats＋saline）, the positive control group （athema rats＋hexadecadrol）, and the YSHXPC decoction treatment group （athema rats＋YSHXPV decoction）. Each group was delivered aerosol inhalation of drug every day since the 14th day. Rats were sacrificed in the 28th day, pathological changes of lung observed by HE staining and TGF β1 expression in lung tissue by immunohistochemieal stain were conducted. Results： The results showed that the TGF β1 expression was higher in normal group compared with other three groups. In YSHXPC decoction group, the TGF β1 expression was reduced significantly compared with the model group and the positive control group. Conclusion： This research showed that YSHXPC decoction can effectively inhibit the TGF β1 expression in experimental rats with asthma.