生姜醇提物对糖尿病小鼠肾损害保护作用的研究

Protective Effect of Zingiber on Kidney Injury Induced by Diabetes in Mice

目的:探讨生姜醇提物对糖尿病小鼠肾损害的保护作用。方法:将30只KM种雄性小鼠随机分为三组,正常对照组(C组,n=10)、模型组(DM组,n=10)及生姜醇提物干预组(ZGB组,n=10)。DM组及ZGB组小鼠尾静脉注射2%四氧嘧啶(90 mg/kg)建立糖尿病肾损害模型。造模成功后,ZGB组给予生姜醇提物溶液0.5 mL(300 mg/kg.d)灌胃14 d,DM组及C组给予生理盐水灌胃。14 d后小鼠断头取血,测定血糖、血清肌酐、尿素氮含量,检测肾组织丙二醛(MDA)及超氧化物歧化酶(SOD)活力的变化,光镜下观察肾组织病理学改变。结果:与DM组比较,ZGB组血糖、血肌酐及尿素氮水平明显低于DM组(P<0.01);肾组织MDA含量明显低于DM组(P<0.01),而T-SOD活力则较DM组升高(P<0.01)。光镜下ZGB组肾脏组织形态学的异常改变较模型组减轻。结论:生姜醇提物对糖尿病小鼠的肾损害有一定的保护作用。

Objective： To investigate the effects of zingiber on kidney injury induced by diabetes in mice. Methods： Thirty Kunming male mice were randomly divided into 3 groups： normal control group （C group, n=10）, diabetic model group （DM group, n=10）, zingiber group （ZGB group, n=10）. The mice in diabetic model and zingiber group were injected 2% alloxan （90 mg/kg） from vena caudalis to establish diabetes models. Once the mice presented with kidney injury, the ZGB group received daily intragastric treatment of ZGB （300 mg/kg＇day）, C group and DM group received daily intragastric treatment of isovolumic normal saline, The animals were executed at 14 days of treatment. The level of blood glucose, urea nitrogen and creatinine in serum were recorded; and the activity of super oxide dismutase （SOD） and malonaldehyde （MDA） content in kidney tissues were measured. The injury circumstance of the kidney tissue in each group was compared by optical microscope. Results： Compared with the DM group, the level of blood glucose, urea nitrogen and creatinine significantly decreased in ZGB group （P〈0.01）; the kidney MDA content decreased, but SOD activity of kidney rose in ZGB group （P〈0.01）. Kidney cell injury in ZGB group was evidently slighter than that of the DM group. Conclusion： Zingiber presented protective effect on kidney injury induced by diabetes in mice.