子宫内膜异位症患者血清、腹腔液、在位/异位内膜组织中RANTES的表达及意义

RANTES Expression in Serum,Peritoneal Fluid and Ectopic/Eutopic Endometrium and Its Clinical Significance in Women with Endometriosis

目的研究子宫内膜异位症(Ems)患者血清、腹腔液、在位/异位内膜组织中RANTES的表达及意义。方法采用酶联免疫吸附(ELISA)法测定研究组(经腹腔镜确诊为盆腔Ems的育龄期妇女,30例)及对照组(因盆腔良性肿瘤行腹腔镜手术、且盆腔结构正常妇女,19例)的血清、腹腔液、在位内膜及异位内膜组织中RANTES的表达,并分析RANTES水平与手术RAS评分、痛经程度的关系。结果血清中RANTES水平:研究组及对照组差异无统计学意义(P=0.350),与手术RAS评分、痛经程度之间无相关性;腹腔液中RANTES水平:研究组与对照组差异有统计学意义(P=0.041),研究组Ⅰ-Ⅱ期与Ⅲ-Ⅳ期差异有统计学意义(P=0.001),与手术RAS评分呈正相关(r=0.764、P=0.000),但与痛经程度无相关性。异位内膜组(n=16)与正常内膜组(n=13)、在位内膜组(n=22)与正常内膜组、异位内膜组与在位内膜组之间RANTES水平差异有统计学意义(P=0.000、P=0.000、P=0.005),在位内膜组、异位内膜组Ⅰ-Ⅱ期与Ⅲ-Ⅳ期RANTES水平差异有统计学意义(P=0.008、P=0.018);异位内膜组和在位内膜组RANTES水平与手术RAS评分呈正相关(r=0.745、P=0.005,r=0.515、P=0.034),但与痛经程度均无相关性。结论 RANTES在Ems患者腹腔液、异位内膜、在位内膜中表达明显增强,且与手术RAS评分呈正相关,与痛经程度无相关性,推测RANTES参与了Ems的发生、发展过程。

Objective To explore the expression of RANTES in serum,peritoneal fluid and ec- topic/eutopic endometrium and its clinical significance in women with endometriosis. Methods The expression of RANTES in serum, peritoneal fluid and ectopic/eutopic endometrium was de- tected by ELISA in 30 women of childbearing age who were diagnosed as having pelvic endometri- osis by laparoseopy （experimental group） and 19 women with normal pelvic structure who under- went laparoscopic surgery for benign pelvic masses （control group）. The relationships of RAN- TES levels to RAS scores and dysmenorrheal degree were analyzed. Results There were no sig- nificant differences in serum RANTES levels between control group and experimental group（P= 0. 350） ,and serum RANTES levels were not correlated with RAS scores and dysmenorrheal de- gree. The differences in peritoneal fluid levels of RANTES were significant between experimental group and control group （P=0. 041） ,as well as between stage Ⅰ-Ⅱand stage Ⅲ-Ⅳ in experi- mental group. RANTES levels in peritoneal fluid were positively correlated with RAS scores（r= 0. 764,P=0. 000）,but were not correlated with dysmenorrheal degree in experimental group. Furthermore,the levels of RANTES were significantly different between ectopic endometrium （n= 16） and normal endometrium （n= 13）, as well as between eutopic endometrium（n = 22） and normal endometrium and between ectopic endometrium and eutopic endometrium（P=0. 000, P= 0. 000 and P=0. 005,respectively）. There was a positive correlation in RANTES levels between stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ in eutopic endometrium and ectopic endometrium（P=0. 008 and P= 0. 018,respectively）. Moreover, RANTES levels in ectopic endometrium and eutopic endome- trium were positively correlated with RAS scores（r=0. 745,P=0. 005 and r=0. 515,P=0. 034, respectively） ,but were not correlated with dysmenorrheal degree. Conclusion The expression of RANTES significantly increases in peritoneal fluid and eutopic/ectopic endometrium from pa- tients with endometriosis, and are positively correlated with RAS scores, but are not correlated with dysmenorrheal degree, suggesting that RANTES plays a important role in the occurrence and development of endometriosis.