重组人NAMPT蛋白静脉注射对正常小鼠生理生化指标及脑结构的影响

Effects of recombinant human NAMPT on physiological/biochemical indexes and brain structure in mice

目的:观察较长时期静脉注射尼克酰胺磷酸核糖转移酶(NAMPT)蛋白对正常小鼠多种生理生化指标及脑结构的影响。方法:32 d内每3天经尾静脉给正常小鼠注射重组野生型NAMPT蛋白或酶活性大大降低的H247A突变型NAMPT蛋白10、30、100μg/kg,检测其体重、血压、心率、血糖、血脂等生理生化指标,观察其脑内海马区神经元、星形胶质细胞、小胶质细胞等的形态。结果:NAMPT野生型和突变型蛋白长期注射对正常小鼠体重、血压、心率、血糖、总胆固醇、甘油三酯等均无明显影响,对其海马CA1区神经、星形胶质细胞和小胶质细胞的形态和数量均未见显著改变。结论:血NAMPT增高可能不是正常个体代谢性疾病及神经系统功能障碍的诱发因素,独立的致病风险较低。

Objective： To observe the effect of recombinant human nicotinamide phosphoribosyl-transferase（NAMPT） on physiological/biochemical indexes and brain structure in mice.Methods： Wild type human recombinant NAMPT（10,30 and 100 μg/kg） or H247A mutant NAMPT（with very weak enzymatic activity） were administrated by intravenous injection in mice once every 3 d for 32 d.The changes of body weight,blood pressure,heart rate,serum glucose,serum total cholesterol and triglyceride were determined,and the morphology of neuron,astrocyte and microglia in hippocampus were observed.Results： The injection of wild and mutated type NAMPT had no significant effect on body weight,blood pressure,heart rate,blood glucose,total cholesterol and triglyceride,and did not affect the morphology of neuron,astrocyte and microglia in hippocampus of mice.Conclusion： Elevation of plasma NAMPT may not induce metabolic and neuronal dysfunction in normal individual.