基于因子分析的肾阳虚证动物模型垂体―靶腺轴关键指标的评价

Evaluation of the Key Indicators in the Pituitary-target Gland Axes in the Animal Model with Shen-yang Deficiency Syndrome Using Factor Analysis

目的评价肾阳虚证动物模型垂体—靶腺轴的关键指标。方法利用因子分析的方法,对垂体—靶腺轴上的8个生物学指标:促甲状腺激素(thyrotropin,thyroid stimulating hormone,TSH)、三碘甲腺原氨酸(3,3',5-triiodothyronine,T3)、四碘甲腺原氨酸(thyroxine,T4)、黄体生成素(luteinizing hormone,LH)、促卵泡激素(follicle-stimulating hormone,FSH)、睾酮(testosterone,T)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)和皮质醇(cortisol,CORT)进行分组,根据自定义判别生物学指标敏感性的敏感度函数,计算各指标的敏感度,进而在各分组内找出敏感度大的指标作为关键指标。结果肾阳虚早期的关键指标为:T、LH、T4和CORT;中期关键指标为:LH、T、CORT和ACTH;晚期关键指标为:LH、T、CORT和FSH。结论 T、LH和CORT是3个时期共有的关键指标,发生变化的关键指标在早、中、晚3个时期分别为T4、ACTH和FSH,即随着时期的不同这一关键指标从甲状腺轴变动到肾上腺轴最后到性腺轴。而且肾阳虚晚期的关键指标主要集中在性腺轴上,表现为大鼠性腺功能全面低下。

Objective To evaluate the key indicators in the pituitary-target gland axes in the ani- mal model of Shen-yang deficiency syndrome （SYDS）. Methods The 8 biological indicators E thyroid stimulating hormone （TSH）, 3, 3＇, 5-triiodothyronine （T3）, thyroxine （ T4 ）, luteinizing hormone （ LH ）, follicle-stimulating hormone （FSH）, testosterone （T）, adrenocorticotropic hormone （ACTH）, and corti- sol （CORT） ] in the pituitary-target gland axes were grouped using factor analysis. Then the sensitivity of every indicator was calculated according to the sensitivity function defined in this paper, so as to find all the most sensitive indicators in every group as key indicators of SYDS. Results The key indicators in the early period of SYDS were T, LH, 3-4, and CORT. The key indicators in the middle period were LH,T, CORT, and ACTH. The key indicators in the late period were LH,T,CORT, and FSH. Conclusions -r, LH, and CORT were the common key indicators of the three periods, and other different key indicator of SYDS in the early, middle and late period were T4, ACTH, and FSH respectively, which changed from the thyroid axis to the adrenal axis and then to the gonadal axis as the period changed. The key indicators in the late period were mainly in the gonadal axis, showing gonadal dysfunction in the late period.