赤芍总苷对D-半乳糖诱导衰老大鼠脑组织TOLL受体和IL-33的实验研究

Effects of Total Paeony Glucosides on mRNA Expressions of Toll Receptors and Interleukin- 33 in the Brain Tissue of D-galactose Induced Aging Rats: an Experimental Research

目的观察赤芍总苷(total paeony glucosides,TPGs)对D-半乳糖(D-galactopyranos,D-gal)诱导衰老大鼠脑组织TOLL受体(Toll like receptors,TLR4)mRNA和白细胞介素-33(interleukin-33,IL-33)mRNA的影响。方法 50只SD大鼠随机分为5组,即空白对照组,模型组,TPG高、中、低剂量组,每组10只。空白对照组给予皮下注射等容量生理盐水,其余40只均皮下注射10%D-gal0.125mL/g,每天1次,连续8周,诱导衰老大鼠模型。从制备模型的第5周开始,对TPG高、中、低剂量组大鼠腹腔注射D-gal同时,分别按300、150和75mg/kg的剂量给予TPG灌胃,每天1次,连续4周。模型组和空白对照组给予灌胃等容量的生理盐水,每天1次。采用Morris水迷宫测定法检测学习记忆能力,RT-PCR检测大鼠脑组织中TLR4mRNA和IL-33mRNA变化。结果与空白对照组比较,模型组大鼠学习记忆能力下降,差异有统计学意义(P<0.05);与模型组比较,各给药组大鼠治疗后学习记忆能力明显增强,并且存在剂量依赖性,差异有统计学意义(P<0.05)。与空白对照组比较,模型组大鼠TLR4mRNA和IL-33mRNA含量明显增多,差异有统计学意义(P<0.05);与模型组比较,各给药组大鼠用药后TLR4 mRNA和IL-33mRNA明显下降,并且存在剂量依赖性,差异均有统计学意义(P<0.05)。结论 TPG通过调节TLR4mRNA和IL-33mRNA的变化,改善D-gal诱导衰老大鼠学习记忆能力。

Objective To investigate effects of total paeony glucosides （TPGs） on the expres- sions of Toll receptors （TLR4） and interleukin-33 （IL-33） in the brain tissue of D-galactose-induced aging rats. Methods Fifty SD rats were randomly divided into 5 groups, i.e., the blank control group, the mod- el group, the high dose TPG group, the middle dose TPG group, and the low dose TPG group, 10 in each group. Equal volume of normal saline was subcutaneously injected to rats in the blank control group, while 10% D-galactose was subcutaneously injected to rats in the rest groups at 0.125 mL/g, once a day for 8 successive weeks to induce the aging rat model. TPG was administered at 300 mg/kg, 150 mg/kg, and 75 mg/kg to rats in the high, middle, and low dose TPG groups while injecting D-galactose from the 5th week of model preparation, once daily for 4 successive weeks. Equal volume of normal saline was ad- ministered to rats in the blank control group and the model group, once daily. The capability for learning and memory was detected using Morris water. The mRNA expressions of TLR4 and IL-33 in the brain tis- sue were detected using ELISA. Results Compared with the blank control group, the capability for learn- ing and memory decreased in the model group with statistical difference （P 〈0.05）. Compared with the model group, the capability for learning and memory was obviously improved in all the medicated groups in a dose-dependent manner, showing statistical difference （P 〈0.05）. Compared with the blank controlgroup, mRNA expressions of TLR4 and IL - 33 in the brain tissue obviously increased after medication in the model group, showing statistical difference （P 〈0.05） . Compared with the model group, mRNA ex- pressions of TLR4 and IL-33 in the brain tissue obviously decreased after medication in all the medicated groups in a dose-dependent manner, showing statistical difference （P 〈0.05）. Conclusion TPGs im- proved D-galactose induced aging rats＇ capability for learning and memory through regulating changes of TLR4 and IL-33 expressions.