E2F1调控细胞周期时程补偿机制的定量研究

A quantitative investigation of E2F1-regulated cell cycle compensation mechanism

目的构建果蝇细胞周期调控的数学模型。方法使用一组常微分方程描述细胞周期网络中核心分子的相互作用,计算分子浓度的连续周期性变化,并捕获分子交互的离散事件。结果计算结果吻合实验观测到的分子浓度的周期变化,揭示和解释了细胞周期时程补偿的数学机制与定量特性。结论 E2F1(E2F transcription factor 1)所联结的正负反馈是调控G1/S与G2/M时程补偿的核心机制。

Objective To explore the core mechanism of cell cycle compensation using a mathematical model. Methods A set of ordinary differential equations were used to describe the interactions between the core cell cycle molecules. Continuous and cyclic changes of the concentrations of these molecules were computed to capture the discrete events of molecular interactions. Results The calculated molecule concentrations and captured signaling events agreed with the experimental results. Conclusion E2F transcription factor 1 is the pivotal element linking the positive and negative feedbacks and regulating G,/S and GdM phase compensation.